摘要
试验旨在研究壳寡糖与干酪乳杆菌对断奶仔猪生长性能、血清中抗氧化酶活性以及肠道微生物菌群结构的影响。试验选用312头30日龄体重为(7.06±0.02)kg杜×长×大三元杂交断奶仔猪随机分成4个处理组,每个处理组设置6个重复,每个重复13头猪。对照组(A)饲喂玉米-豆粕型基础日粮、试验组(B)每千克饲料添加0.5 g壳寡糖、试验组(C)每千克饲料添加1010 CFU干酪乳杆菌、试验组(D)同时添加壳寡糖与干酪乳杆菌,试验期为32 d。结果表明:D组有提高断奶仔猪0~14、15~32 d和0~32 d这三个阶段平均日增重和降低料重比的作用,其中15~32 d阶段料重比降低效果显著(P<0.05)。试验14 d,C、D组中弗林氏丁酸杆菌(Flintibacter butyricum)与卷曲乳杆菌(Lactobacillus crispatus)较A、B组明显增多。试验32 d,B组显著提高了血清中谷胱甘肽过氧化物酶(GSH-Px)的含量(P<0.05),并降低了丙二醛(MDA)的含量(P>0.05)。C组降低了断奶仔猪的腹泻率。C、D组中布氏瘤胃球菌(Ruminococcus bromii)和罗氏弧菌(Roseburia intestinalis)的数量明显高于A、B组。壳寡糖作为饲料添加剂可提高断奶仔猪机体抗氧化能力;乳酸菌则可提高肠道益生菌数量降低断奶仔猪腹泻率;同时添加壳寡糖和乳酸菌可以有效提高断奶仔猪的饲料转化率,提高肠道有益微生物数量,使仔猪肠道更健康。
The study was carried out to determine the effects of chitosan oligosaccharide(COS)and Lactobacillus casei(L.casei)supplementation on growth performance,serum antioxidant enzyme activi⁃ty and structure of intestinal microflora of weanling piglets.A total of 312 crossed-bred(Duroc×Changbai×Dabai)weanling piglets,weighted an average body weight of(7.06±0.02)kg,were randomly divided into 4 treatments with 6 replicates and 13 piglets per treatment according to the body weight:control group(A)fed a basal diet,groups(B,C,D,E)fed experimental diets supplemented with 0.5 g/kg COS,1010 CFU/kgL.casei,0.5 g/kg COS and 1010 CFU/kgL.casei respectively.Supplemen⁃tation of 0.5 g/kg COS and 1010 CFU/kgL.casei(group D)increased the average daily gain(ADG)and reduced the feed to gain ration(F/G)for the periods 0~14 d,15~32 d and 0~32 d,especially the effect of reducing F/G for the period 15~32 d was remarkable(P<0.05).Supplementation of 1010 CFU/kg L.casei(group C)decreased ratio of diarrhoea of weanling piglets.Supplementation of 0.5 g/kg COS(group B)increased the activities of glutathione peroxidase(GSH-Px)and reduced the concentra⁃tion of malondialdehyde(MDA)in serum for the period 0~32 d(P<0.05).After 14 days,the quan⁃tity of Flintibacter butyricum and Lactobacillus crispatus of group C and D are higher than group A and B,meanwhile,the quantity ofRuminococcus bromii andRoseburia intestinalis of group C and D are higher than group A and B after 32 days.In conclusion,COS could improve antioxidant function,L.casei could increase the number of intestinal probiotics and decrease ratio of diarrhoea of weanling piglets;Supplementation of COS and L.casei could improve feed conversion rate,increase the number of beneficial microorganisms in intestinal tract and make the intestine healthier of piglets.
作者
刘媛媛
解林奇
吴栋
李康
陈浩天
宋维平
闫雪
Liu Yuanyuan;Xie Linqi;Wu Dong;Li Kang;Chen Haotian;Song Weiping;Yan Xue
出处
《饲料工业》
北大核心
2019年第24期25-31,共7页
Feed Industry
基金
国家重点研发计划项目[2017YFD0501006]
关键词
壳寡糖
干酪乳杆菌
断奶仔猪
生长性能
抗氧化指标
肠道微生物
chitosan oligosaccharide
Lactobacillus casei
weanling piglets
growth performance
antioxi⁃dant indices
intestinal flora