摘要
目的 探讨何氏养巢颗粒对小鼠卵巢储备功能下降(DOR)的防治作用.方法 取动情周期正常ICR雌鼠分为对照组、模型组、DHEA组及养巢颗粒低、中、高剂量组,每组各10只,除对照组外其他组均采用环磷酰胺(CTX)诱导DOR小鼠模型,分别予生理盐水及DHEA、养巢颗粒灌胃8周.阴道涂片观察动情周期,分时段检测血清促卵泡刺激素(FSH)、促黄体生成素(LH)、雌二醇(E2)、抗苗勒管激素(AMH)、抑制素B(INHB),解剖后计算卵巢、子宫系数,光镜下观察卵巢形态组织学变化.结果 与对照组比较,模型组小鼠动情周期延长,FSH、LH值升高且AMH下降(P<0.05);与模型组比较,DHEA组、养巢颗粒各剂量组FSH值下降,E2、INHB值升高,高剂量组AMH显著增高(P<0.05);相较于第4周,第8周模型组及DHEA组的FSH值较前明显升高(P<0.05),而养巢颗粒组小鼠的FSH、LH较前次检测无明显变化(P>0.05).观察卵巢组织形态可见相较于模型组,DHEA组及养巢颗粒各组均可见卵巢中各级卵泡数目增多且闭锁卵泡减少.结论 何氏养巢颗粒能够调节性激素水平、减缓卵泡闭锁,对改善卵巢储备功能具有良好的效果,能够及时防治DOR的进一步发展.
Objective To study the preventive and therapeutic effects of He’s Yangchao Decoction on ovaries of rats with diminished ovarian reserve(DOR).Methods ICR female rats with normal estrus cycle were divided into control group,model group,DHEA group,and low-,medium-,and high-dose groups of Yangchao Decoction,with 10 rats in each group.All other groups except the control group were treated with cyclophosphamide(CTX).Saline,DHEA(11mg·kg^-1),low dose(2.4g·kg^-1),medium dose(4.8g·kg^-1)and high dose(9.6g·kg^-1)of Yangchao Decoction were administered for 8 weeks.Vaginal smear was used to observe estrous cycle.Two times of serum follicle stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),anti-Mullerian hormone(AMH),and inhibin B were detected.The ovarian and uterus coefficients were calculated after dissection,and the morphology of ovary and the growth and development of follicles were observed under light microscope.Results Compared with the control group,the estrous cycle of the model group was prolonged,the FSH and LH values were increased,and the AMH was decreased(P<0.05).Compared with the model group,the FSH values of the DHEA group and the Yangchao Decoction decreased,E2 and INHB value increased,the LH value of the medium and high dose Yangchao Decoction group decreased,and the AMH of the high dose group increased significantly(P<0.05).The FSH values of the model group and the DHEA group were significantly higher than before(P<0.05),while the FSH and LH values of the Yangchao Decoction group had no significant change compared with the previous one(P>0.05).The morphology of ovarian tissue was observed,and compared with the model group,the number of follicles in the ovary increased and the number of atretic follicles decreased in the DHEA group and the Yangchao Decoction group.Conclusion He's Yangchao Decoction can regulate the level of sex hormones,slow down follicular atresia,and has a good effect on improving ovarian reserve function,and can prevent and control the further development of DOR.
出处
《浙江临床医学》
2019年第11期1479-1481,共3页
Zhejiang Clinical Medical Journal
基金
浙江省中医药科技重点计划项目(2017ZW004)。
关键词
何氏养巢颗粒
卵巢储备功能下降
环磷酰胺
He’s Yangchao decoction
Diminished ovarian reserve
Cyclophosphamide
