摘要
目的探讨双调蛋白(amphiregulin,AREG)在不同程度嗜酸粒细胞浸润的鼻息肉中的表达差异,并分析其与鼻息肉组织重塑的相关性。方法纳入2017年7月至2018年8月在武汉大学人民医院耳鼻咽喉头颈外科行鼻内镜手术的48例患者,其中男性40例,女性8例,年龄16~60岁。研究对象分为3组:对照组14例、嗜酸粒细胞型慢性鼻窦炎伴鼻息肉(eosinophilic chronic rhinosinusitis with nosal polyps,ECRSwNP)组19例、非嗜酸粒细胞型慢性鼻窦炎伴鼻息肉(noneosinophilic chronic rhinosinusitis with nosal polyps,non-ECRSwNP)组15例。通过蛋白免疫印迹法(Western blot)和免疫组织化学法检测AREG在鼻黏膜组织中的相对表达量。采用苏木精-伊红(HE)染色、阿利新蓝-过碘酸-雪夫(AB-PAS)染色、马松三色胶原(MT)染色检测组织重塑情况。多组间比较采用K-W检验;将CRSwNP患者鼻黏膜中AREG的蛋白表达水平分别与组织重塑相关指标做Spearman相关性分析。结果ECRSwNP组的AREG表达水平显著高于non-ECRSwNP组和对照组(Western blot蛋白表达中位数1.592比0.617比0.582,P值均<0.05)。ECRSwNP组的黏膜上皮损伤程度和杯状细胞化生程度显著高于对照组(P值均<0.05),ECRSwNP组的胶原纤维面积百分比显著低于对照组(P=0.01)。慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者中黏液腺体面积与AREG表达呈负相关(r=-0.616,P<0.05),胶原纤维面积百分比与AREG表达呈负相关(r=-0.738,P<0.05)。结论AREG在ECRSwNP中的表达水平较高,并与鼻息肉组织重塑过程相关。
Objective To explore the expression of amphiregulin(AREG)in nasal polyps patients with different degrees of eosinophil infiltration,and to analyze the correlation between AREG and tissue remodeling.Methods Forty-eight patients underwent endoscopic sinus surgery in the Department of Otorhinolaryngology Head and Neck Surgery,Remin Hospital,Wuhan University from July 2017 to August 2018 were recruited,including 40 males and 8 females,aged from 16 to 60 years old.The subjects were divided into three groups:control group(n=14),eosinophilic chronic sinusitis with nasal polyps(ECRSwNP)group(n=19)and noneosinophilic chronic rhinosinusitis with nasal polyps(non-ECRSwNP)group(n=15).The relative expression of AREG in nasal mucosa was detected by Western blot assay and immunohistochemical staining.Tissue remodeling was detected by HE staining,AB-PAS staining and Masson staining.Kruskal-Wallis test was used for comparison among multiple groups,and Spearman correlation analysis was conducted between the expression level of AREG and the related indexes of tissue remodeling.Results The expression of AREG in ECRSwNP group was significantly higher than that in non-ECRSwNP group and control group(median protein expression of Western blot was 1.592 vs 0.617 vs0.582,all P<0.05).The degree of epithelial injury and goblet cell metaplasia in ECRSwNP group was significantly higher than that in control group(all P<0.05),the percentage of collagen fibrosis area in ECRSwNP group was significantly lower than that in control group(P=0.01).In chronic rhinosinusitis with nasal polyps(CRSwNP)patients,the area of mucous glands was negatively correlated with the expression of AREG(r=-0.616,P<0.05),and the percentage of collagen fibrosis area was negatively correlated with the expression of AREG(r=-0.738,P<0.05).Conclusion The expression of AREG is higher in ECRSwNP patients,which is related to the process of tissue remodeling.
作者
高玲
许昱
章薇
向荣
姚冲
孔勇刚
陶泽璋
Gao Ling;Xu Yu;Zhang Wei;Xiang Rong;Yao Chong;Kong Yonggang;Tao Zezhang(Department of Otorhinolaryngology Head and Neck Surgery,Renmin Hospital,Wuhan University,Wuhan 430060,China)
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2019年第11期850-856,共7页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
国家自然科学基金(81770986)。
