摘要
采用顶空固相微萃取法结合手性气相色谱-质谱联用技术对不同花色福鼎白茶的香气成分进行分析,得出白毫银针、白牡丹及寿眉样品中14 种挥发性萜类化合物的对映异构体分布情况。相对定量分析结果表明,S-芳樟醇、S-Z-橙花叔醇及(2S,5R)-芳樟醇氧化物B在大部分白茶样品中具有较高的相对含量,而茶螺烷的4 个对映异构体的相对含量普遍较低。对映异构体比例(enantiomeric ratio,ER)分析结果表明,S-柠檬烯、(2S,5S)-芳樟醇氧化物A、(2S,5R)-芳樟醇氧化物B、S-芳樟醇、(2R,5R)-茶螺烷A、(2R,5S)-茶螺烷B、R-4-萜品醇、S-α-松油醇、S-香茅醇、S-α-紫罗兰酮、S-Z-橙花叔醇、S-E-橙花叔醇、(1R,2R)-茉莉酸甲酯及(1R,2S)-茉莉酸甲酯是大部分福鼎白茶中相应萜类化合物的主导立体构型,其中芳樟醇、茶螺烷B、4-萜品醇、香茅醇、α-紫罗兰酮、芳樟醇氧化物A及Z-橙花叔醇的ER值与福鼎白茶的采摘嫩度呈现一定的相关性。多元统计分析结果表明,12 个萜类化合物对映异构体的含量在不同花色福鼎白茶中存在显著差异,其中R-芳樟醇、(2R,5S)-茶螺烷B、S-α-松油醇、R-E-橙花叔醇、R-Z-橙花叔醇、S-柠檬烯及S-E-橙花叔醇在白毫银针样本中含量最高,(2R,5R)-芳樟醇氧化物A及(2S,5R)-茶螺烷B含量与白毫银针样本呈负相关;S-Z-橙花叔醇及(1S,2S)-茉莉酸甲酯在白牡丹样本中含量普遍较高;而R-α-紫罗兰酮在寿眉样本中有较高的含量分布。本研究为后续白茶香气品质形成机理研究、白茶花色等级判别及指纹图谱构建等提供理论依据。
In this work, the enantiomeric distribution of 14 volatile terpenoids in different types of Fuding white teaswere analyzed by headspace-solid-phase microextraction (HS-SPME) and enantioselective gas chromatography-massspectrometry (GC-MS). The relative quantitative analysis results showed that S-linalool, S-Z-nerolidol and (2S,5R)-linalooloxide B were relatively more abundant in most white teas, while the contents of four enantiomers of theaspirane wereextremely low. The enantiomeric analysis results indicated that S-limonene, (2S,5S)-linalool oxide A, (2S,5R)-linalooloxide B, S-linalool, (2R,5R)-theaspirane A, (2R,5S)-theaspirane B, R-terpinen-4-ol, S-α-terpineol, S-citronellol, S-α-ionone,S-Z-nerolidol, S-E-nerolidol, (1R,2R)-methyl jasmonate and (1R,2S)-methyl epijasmonate were the major absoluteconfigurations of terpenes in most white teas, and that the enantiomeric ratios (ERs) of linalool, linalool oxide B, terpinen-4-ol, citronellol, α-ionone, linalool oxide A and Z-nerolidol were related to the plucking standard of white teas. Moreover, multivariate statistical analysis exhibited that a significant difference in the contents of 12 volatile terpenoid enantiomersexisted among the three different types of Fuding white teas. R-Linalool, (2R,5S)-theaspirane B, S-α-terpineol, R-E-nerolidol,R-Z-nerolidol, S-limonene and S-E-nerolidol presented the highest levels in Silver Needle tea samples, while the oppositewas true for (2R,5R)-linalool oxide A and (2S,5R)-theaspirane B. S-Z-Nerolidol and (1S,2S)-methyl epijasmonate showedhigher contents in White Peony tea samples, and the content of R-α-ionone was higher in Shou Mei tea samples than in theother types. The above studies provide the theoretical foundation for further understanding the formation mechanism of thearoma quality of, discriminating the types and grades of and establishing a fingerprint for white teas.
作者
严寒
朱荫
穆兵
吕海鹏
KANG Suyoung
张悦
林智
YAN Han;ZHU Yin;MU Bing;LÜHaipeng;KANG Suyoung;ZHANG Yue;LIN Zhi(Key Laboratory of Tea Biology and Resource Utilization,Ministry of Agriculture,Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第24期193-200,共8页
Food Science
基金
国家自然科学基金青年科学基金项目（31701702）
中国农业科学院创新工程项目（CAAS-ASTIP-2014-TRICAAS）
现代农业产业技术体系建设专项（CARS-19）
