摘要
采用固相微萃取(Solid-Phase Microextraction,SPME)与溶剂辅助风味蒸发(Solvent-Assisted Flavor Evaporative,SAFE)法结合香气活性值(Odor Activity Value,OAV)和气相色谱-嗅闻-质谱联用技术(Gas Chromatography-Olfactory-Mass Spectrometry,GC-O-MS)分析鉴定新鲜香椿中关键香气成分。结果显示,采用两种萃取方法共鉴定出90种挥发性化合物,主要包括含硫类12种,醛类11种,萜烯类34种,酮类2种,醇类4种,酯类4种,酚类1种,酸类1种,烷烃类15种,环烷烃类4种,其他类2种。经OAV值计算,共确定出(E,E)/(E,Z)/(Z,Z)-二丙烯基二硫醚、反-2-巯基-3,4-二甲基-2,3-二氢噻吩、2-甲基环硫乙烷、己醛、3-甲基-正丁醛、反-2-己烯醛、反-2-辛烯醛等9种香气成分;经GC-O-MS分析,共鉴定出(E,E)/(E,Z)/(Z,Z)-二丙烯基二硫醚、2-甲基环硫乙烷、反-2-巯基-3,4-二甲基-2,3-二氢噻吩、己醛、反-2-己烯醛等7种香气活性物质。综合OAV和GC-O-MS分析,最终确认(E,E)/(E,Z)/(Z,Z)-二丙烯基二硫醚、反-2-巯基-3,4-二甲基-2,3-二氢噻吩、2-甲基环硫乙烷、己醛、反-2-己烯醛为新鲜香椿的关键香气成分。
The key aroma components in fresh Toona sinensis were analyzed and identified using solid-phase microextraction and solvent assisted flavor evaporation,in combination with odor activity value(OAV)and gas chromatography olfactory mass spectrometry(GC-O-MS).The two extraction methods identified 90 volatile compounds,including 12 sulfur compounds,11 aldehydes,34 terpenes,2 ketones,4 alcohols,4 esters,1 phenol,1 acid,15 alkanes,4 cycloalkanes,and 2 other compounds.Through OAV calculation,nine aroma active components were identified,including(E,E)-,(E,Z)-,and(Z,Z)-bis(1-propenyl)disulfide,trans-2-mercapto-3,4-dimethyl-2,3-dihydrothiophene,methyl-thiirane,hexanal,3-methyl-butanal,(E)-2-hexanal,and(E)-2-octenal.Seven aroma active compounds were identified using GC-O-MS,including(E,E)-,(E,Z)-,and(Z,Z)-bis(1-propenyl)disulfide,trans-2-mercapto-3,4-dimethyl-2,3-dihydrothiophene,methyl-thiirane,hexanal,and(E)-2-hexanal.The OAV and GC-O-MS analyses confirmed that(E,E)-,(E,Z)-,and(Z,Z)-bis(1-propenyl)disulfide,trans-2-mercapto-3,4-dimethyl-2,3-dihydrothiophene,methyl-thiirane,hexanal,and(E)-2-hexanal were the key aroma components in fresh Toona sinensis.
作者
赵丽丽
史冠莹
蒋鹏飞
张乐
王旭增
王赵改
ZHAO Lili;SHI Guanying;JIANG Pengfei;ZHANG Le;WANG Xuzeng;WANG Zhaogai(Agricultural Products Processing Center,Henan Academy of Agricultural Sciences,Zhengzhou 450000,China)
出处
《现代食品科技》
CAS
北大核心
2022年第11期264-275,共12页
Modern Food Science and Technology
基金
科技部科技伙伴计划资助项目(KY202002007)
河南省农业科学院新兴学科发展专项(2020XK08,2021XK01,2022XK01)
河南省农业科学院优秀青年科技基金项目(2020YQ37)
河南省科技攻关项目(212102110018)
河南省农业科学院自主创新专项(2022ZC59)。
关键词
香椿
香气
固相微萃取
溶剂辅助风味蒸发
香气活性值
气相色谱-嗅闻-质谱
Toona sinensis
aroma
solid phase microextraction
solvent assisted flavor evaporation
odor activity value
gas chromatography-olfactory-mass spectrometry
