摘要
目的建立貂心质量标准。方法对4个产地11批样品进行TLC定性鉴别、聚合酶链式反应,药典法测定氮、水分、总灰分、重金属及有害物质(Pb、As、Cr、Hg、Cu)含有量。结果TLC斑点清晰,可将正品与非动物类伪品区分开;300~400bp处有单一DNA条带扩增,可鉴别出基源性伪品。氮在0.1~1.0g范围内线性关系良好(r=0.9999),平均加样回收率为964%,RSD为0.63%。氮、水分、总灰分平均含有量分别为11.56%、7.02%、3.76%,重金属及有害物质含有量符合国家标准。结论该方法稳定可靠,可用于貂心的质量控制。
AIM To establish the quality standard for Mustela vison Schreber hearts.METHODS For eleven batches of samples from four producing areas,TLC qualitative identification and polymerase chain reaction were performed,and pharmacopeia method was adopted in the content determination of nitrogen,water,total ash,heavy metals and hazardous substances(Pb,As,Cr,Hg,Cu).RESULTS The clear TLC spots could distin guish certified products from non animal counterfeits,and the single DNA band amplification at 300-400 bp could identify original counterfeits.Nitrogen showed a good linear relationship within the range of 01-10 g(r=09999),whose average recovery was 964%with the RSD of 063%.The average contents of nitrogen,water and total ash were 1156%,702%and 376%,respectively,and those of heavy metals and hazardous substances accorded with national standards.CONCLUSION This stable and reliable method can be used for the quality control of M.vison hearts.
作者
崔馨元
王艳双
李盈诺
张丽华
苑广信
CUI Xin⁃yuan;WANG Yan⁃shuang;LI Ying⁃nuo;ZHANG Li⁃hua;YUAN Guang⁃xin∗(College of Pharmacy,Beihua University,Jilin 132013,China;College of Medicine,Beihua University,Jilin 132013,China;Jilin Municipal Leibo Technology Co.,Ltd.,Jilin 132013,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第12期2858-2862,共5页
Chinese Traditional Patent Medicine
基金
长白山药用动植物活性多肽国家地方联合工程实验室(2015)
吉林省中药材地方标准项目(JLBZ201708)
吉林省产业技术研究与开发专项(2018C048-2)
关键词
水貂
心脏
氮
水分
总灰分
TLC
聚合酶链式反应
药典法
Mustela vison Schreber
hearts
nitrogen
water
total ash
TLC
polymerase chain reaction
pharmacopeia method
