摘要
目的环磷腺苷效应元件结合蛋白1(cAMP responsive element binding protein1,CREB1)是真核细胞中重要的转录调节因子,与多种细胞信号通路有关并参与多种肿瘤的进展。本研究检测CREB1在进展期胃腺癌组织中的表达,分析其与进展期胃腺癌临床病理特征之间的关系。方法选取2010-01-01-2012-12-30青岛大学附属医院行手术切除的92例进展期胃腺癌及相应癌旁正常组织(距癌组织>5cm)的蜡块制备组织芯片。92例癌旁正常组织因实验中脱片等因素造成不合格病例17例,剩余75例癌旁正常组织纳入分析。采用免疫组织化学方法检测CREB1在进展期胃腺癌组织中表达情况。结果 92例进展期胃腺癌组织中41例(44.6%)CREB1高表达,75例癌旁正常组织中仅4例(5.3%)CREB1呈高表达,差异有统计学意义,(χ^2=32.305,P<0.001)。CREB1在进展期胃腺癌中的表达与浸润深度(χ^2=4.378,P=0.036)、TNM分期(χ^2=4.168,P=0.041)和脉管侵犯(χ^2=8.802,P=0.003)有关联。CREB1高表达的进展期胃腺癌患者总生存率(χ^2=6.790,P=0.009)和无病生存率(χ^2=6.418,P=0.011)低于CREB1低表达患者,差异有统计学意义;Cox多因素分析结果显示,CREB1表达是影响进展期胃腺癌患者预后的独立危险因素。结论 CREB1可能参与了胃腺癌的发生与发展;CREB1的检测可能为进展期胃腺癌患者的预后判断提供依据,并可能成为胃腺癌患者靶向治疗的新靶点。
OBJECTIVE CAMP responsive element binding protein1(CREB1)is an important transcriptional regulator in eukaryotic cells,which is related to a variety of cellular signaling pathways and participates in the evolution of various tumors.The aim of this study was to investigate the expression of CREB1 in advanced gastric adenocarcinoma and analyze its relationship with clinicopathologic features of advanced gastric adenocarcinoma.METHODS Tissue chip was constructed based on wax blocks of 92 advanced gastric adenocarcinomas and the corresponding adjacent normal tissues(5 cm from cancerous tissue)from Affiliated Hospital of Qingdao University,between January 1,2010 and December 30,2012.Seventeen cases were excluded due to experimental de-fragmentation and other factors,the remaining 75 cases were put into analysis.The expression of CREB1 was detected in advanced gastric adenocarcinoma by immunohistochemistry.RESULTS Of 92 advanced gastric adenocarcinoma tissues,41(44.6%)had high expression of CREB1.Of 75 corresponding adjacent normal tissues,4(5.3%)had high expression of CREB1.The high expression of CREB1 in advanced gastric adenocarcinoma tissues was higher than that in adjacent normal tissues(χ^2=32.305,P<0.001),the difference was statistically significant.The expression of CREB1 was correlated to the depth of invasion(χ^2=4.378,P=0.036),TNM grade(χ^2=4.168,P=0.041)and vascular invasion(χ^2=8.802,P=0.003).Overall survival(χ^2=6.790,P=0.009)and disease free survival(χ^2=6.418,P=0.011)of high CREB1 expression were lower than low CREB1 expression in advanced gastric adenocarcinoma patients,the difference was statistically significant.CREB1 expression was an independent risk factor for the prognosis of advanced gastric adenocarcinoma patients according to multivariate analysis.CONCLUSIONS CREB1 may be involved in the occurrence and development of gastric adenocarcinoma.Detection of CREB1 expression may have important prognostic values in advanced gastric adenocarcinoma.CREB1 may become a new target for targeted thera
作者
姜萍
谭真
周璇
李宏
项锋钢
JIANG Ping;TAN Zhen;ZHOU Xuan;LI Hong;XIANG Feng-gang(Department of Pathology,School of Basic Medical Science,Qingdao University,Qingdao 266021,P.R.China;Department of Pathology f Third Peoples Hospital of Linyi,Linyi 276023,P.R.China;Department of Pathology A.ffiliated Hospital of Qingdao University,Qingdao 266003,P.R.China)
出处
《中华肿瘤防治杂志》
CAS
北大核心
2019年第20期1531-1536,共6页
Chinese Journal of Cancer Prevention and Treatment
关键词
进展期胃腺癌
CREB1
组织芯片
免疫组化
预后
advanced gastric adenocarcinoma
CREB1
tissue microarray
immunohistochemistry
prognosis
