摘要
目的采用超高效液相色谱结合高分辨率质谱(UPLC-Q-TOF-MS)技术观察高脂血症大鼠造模后血浆内源性代谢物的差异情况,并寻找潜在的生物标志物。方法将SD大鼠分成正常对照组和模型组,运用高脂高胆固醇饲料连续喂养4周复制高脂血症大鼠模型。眼眶采血收集血样,采用UPLC-Q-TOF-MS检测并进行主成分分析。结果高脂血症模型被成功复制。与正常对照组相比,模型组大鼠血浆溶血磷脂的含量发生明显改变,如LysoPC(20∶5(5Z,8Z,11Z,14Z,17Z)),LysoP C(22∶5(7Z,10Z,13Z,16Z,19Z),LysoPC(20∶3(5Z,8Z,11Z)),LysoPC(18∶3(6Z,9Z,12Z)),L ysoPC(22∶5(7Z,10Z,13Z,16Z,19Z)。结论高脂血症大鼠血浆脂质代谢发生了紊乱。
OBJECTIVE To identify the protential biomarkers,ultra-perform ance liquid chromatography(UPLC)coupled with quadrupole time-of-flight mass spectrometry(Q-TOF-MS),a metabonomics method,was used to study the metabolic changes in the plasma of hyperlipidemia rat.METHODS SD rats were divided into normal group and model group.This model of hyperlipidemia was established by high fat and cholesterol diet for 4 weeks.Plasma samples were collected from the orbital sinus,and UPLC-TOF-MS was used for the principal component analysis.RESULTS The hyperlipidemia rat model was replicated successfully.Compared with the normal group,plasma substances such as LysoPC(20∶5(5Z,8Z,11Z,14Z,17Z)),LysoPC(22∶5(7Z,10Z,13Z,16Z,19Z)),LysoPC(20∶3(5Z,8Z,11Z)),LysoPC(18∶3(6Z,9Z,12Z),LysoPC(22∶5(7Z,10Z,13Z,16Z,19Z))were obviously changed in the model group.CONCLUSION The results in this study suggested that the lipid metabolisms are significantly disturbed.
作者
李瑞鹏
欧慧瑜
郭秋平
倪庆纯
LI Rui-Peng;OU Hui-yu;GUO Qiu-ping;NI Qing-chun(Drug nonclinical evaluation and research center of Guangzhou pharmaceutical research institute,Guangzhou 510240,China)
出处
《海峡药学》
2019年第11期24-26,共3页
Strait Pharmaceutical Journal
基金
广州市科信局生物产业重大专项(编号:201300000053)
