摘要
目的:研究血必净对重症急性胰腺炎(SAP)大鼠肝损伤的保护作用及其对肝脏信号传导与转录激活因子3(p-STAT3)表达的影响。方法:60只6~8周龄清洁级雄性SD大鼠按照随机数字表法分成3组:胰腺炎模型组、假手术组、血必净组,每组20只。胰腺炎模型组和血必净组采用5%牛磺胆酸钠逆行胰胆管注射制备重症SAP大鼠模型,假手术组打开腹腔后不注射药物,血必净组在造模后腹腔内1次性注射血必净4 mL/kg。造模成功后12、24 h,3组随机各取大鼠10只,心脏穿刺取血5 mL,应用全自动生化分析仪检测血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST),ELISA法检测血清IL-6、TNF-α水平,Western blotting法检测肝脏p-STAT3蛋白表达,取血后处死,取一叶肝组织制作石蜡切片,进行HE染色和免疫组化染色,观察肝组织损伤程度及p-STAT3蛋白表达情况。结果:胰腺炎模型组和血必净组大鼠ALT、AST水平显著升高,炎性因子IL-6、TNF-α及p-STAT3蛋白表达较假手术组均明显升高(均P<0.05),血必净组转氨酶ALT、AST水平、IL-6、TNF-α、p-STAT3蛋白表达水平均较胰腺炎模型组低(均P<0.05)。结论:血必净对大鼠SAP肝损伤有保护作用,可能与调控p-STAT3信号通路,抑制促炎细胞因子过度表达有关。
Objective: To investigate the effect of Xuebijing on severe acute pancreatitis(SAP)-associated acute liver injury in the rats and explore the underlying mechanisms. Methods: Male Sprague-Dawley(SD) rats(n=60) were randomly divided into sham-operated group(n=20), SAP model group(n=20), and treatment group(n=20). SAP model was induced by retrograde injection of 5% sodium taurocholate(1 mL/kg) into the biliopancreatic duct. The rats in Xuebijing treatment group were intraperitoneally injected with 4 mL/kg Xuebijing. The rats were sacrificed at 12 h(n=10) and 24 h(n=10) after the treatment. The levels of serum ALT and AST were determined by the puncture through inferior vena cava. The serum levels of TNF-α and IL-6 were examined by ELISA, and the protein levels of p-STAT3 in the liver were evaluated by Western blotting. Results: As compared with sham-operated group,the levels of serum ALT, AST, IL-6 and TNF-α in SAP model group were significantly increased, and those in Xuebijing treatment group were significantly decreased as compared with those in model group. Meanwhile, the phosphorylation levels of p-STAT3 were significantly increased in model group as compared with sham-operated group. The rats in Xuebijing treatment group had lower phosphorylation levels of STAT3. Conclusion: Xuebijing exerts a protective effect on pancreatitis-associated acute liver injury in the rats possibly via inhibiting p-STAT3 signaling pathway.
作者
王晓君
陈曾凤
方兴刚
WANG Xiao-jun;CHEN Zeng-feng;FANG Xing-gang(Tailie Hospital of Shiyan City,Hubei Province,Shiyan 442000 China)
出处
《内科急危重症杂志》
2019年第5期418-420,共3页
Journal of Critical Care In Internal Medicine
基金
湖北省教育厅科研项目(No:B2016116)
