摘要
目的探讨Xpert MTB/RIF快速检测在肺结核患者诊断中的应用价值。方法应用Xpert MTB/RIF快速检测技术对106例住院的肺结核患者和疑似肺结核患者晨痰标本进行检测,同时做抗酸染色、全血γ-干扰素释放试验和血清结核杆菌IgG抗体检测。将Xpert MTB/RIF快速检测分别与抗酸染色、γ-干扰素释放试验、结核杆菌IgG抗体检测的阳性率进行比较,将Xpert MTB/RIF与抗酸染色的结果进行对比分析。结果晨痰标本采用Xpert MTB/RIF快速检测技术,MTB阳性率为68.87%(73/106),其中利福平耐药14例,耐药率为19.18%(14/73).抗酸染色阳性率为、全血γ-干扰素释放试验、结核杆菌IgG抗体阳性率分别为24.53%(26/106)、62.26%(66/106)、28.30%(30/106)。Xpert MTB/RIF的阳性率高于抗酸染色、结核杆菌IgG抗体检测,差异均有统计学意义(χ^2=41.86、34.92,P<0.05)。结论Xpert MTB/RIF检测速度快,操作简单,在肺结核患者快速诊断中优于其他三种方法。
Objective To investigate the application value of Xpert MTB/RIF rapid detection in the diagnosis of pulmonary tuberculosis.Methods The rapid detection technique of Xpert MTB/RIF was used to detect the morning sputum samples of 106 hospitalized and suspected pulmonary tuberculosis patients.At the same time,acid-fast stain test,whole-blood interferon-γrelease assay and serum Mycobacterium tuberculosis antibody test were performed.The positive rates of Xpert MTB/RIF were compared with those of other three tests,and the test result of Xpert MTB/RIF were compaed with acid-fast stain test.Results The positive rate of MTB was 68.87%(73/106),14 of which were rifampicin resistant accounting for 19.18%(14/73).The positive rates of acid-fast stain test,whole-blood interferon-γrelease assay and Mycobacterium tuberculosis antibody test were 24.53%(26/106),62.26%(66/106)and 2830%(30/106).The positive rate of Xpert MTB/RIF was higher than acid-fast stain test and Mycobacterium tuberculosis antibody test(χ2=41.86,34.92,P<0.05).Conclusion Xpert MTB/RIF detection is fast and easy to operate,which is superior to the other three methods in the rapid diagnosis of tuberculosis.
作者
钟莉
许铠
ZHONG Li;XU Kai(Department of Clinical Laboratory,the First People′s Hospital of Honghe State,Mengzi,Yunnan 661199,China)
出处
《国际检验医学杂志》
CAS
2019年第23期2887-2889,共3页
International Journal of Laboratory Medicine
