摘要
目的探讨建立C57BL/6J小鼠急性前庭损伤动物模型的方法及其行为学评估。方法健康成年雄性C57BL/6J小鼠(20~30g)共21只,随机分成单侧迷路切除术组(n=15)及假手术组(n=6)两组。单侧迷路切除术组小鼠,在麻醉后在显微镜下进行其左耳的单侧迷路切除术(UL),行耳前切口,钝性分离组织,勾除鼓膜、锤骨、砧骨,电凝镫骨动脉,勾除镫骨,打开镫骨底板,暴露前庭池,用吸引器吸除流出的内淋巴液,用精细的勾针分离椭圆囊及球囊,并用勾针破坏该区域剩余的前庭神经上皮细胞及半规管,最后往前庭内塞入含有无水乙醇的明胶海绵,缝合皮肤。而假手术组小鼠,通过耳前切口打开其外耳道,缝合皮肤。对所有术后小鼠进行行为学观察,并在术后第10天进行爬杆试验、平衡木试验的行为学检测。结果所有单侧迷路切除术后小鼠在清醒后均开始出现一种特殊的偏斜姿势,头右偏,走路时向右转圈,身体筒状翻滚,提尾时出现以身体为轴线的旋转。以上体征在一周内逐渐缓解。术后第10天,手术组跟假手术组相比,爬杆试验(P=0.000)及平衡术试验(P=0.002)的结果均具有统计学差异(P<均0.01)。结论使用单侧迷路切除术(UL)配合化学切除的方法能建立稳定、有效、造模成功率高的急性前庭损伤小鼠模型。在术后一周时,可观察到小鼠的部分静态症状明显缓解。而行为学检测结果提示在术后第10天,前庭损伤小鼠模型尚未完全完成前庭代偿。
Objective To establish and assess the validity of a model of acute vestibular injury using C57 BL/6 J mise. Methods Twenty-one adult male mice were randomly divided into a surgery(n=15) and a sham surgery group(n=6). In the surgery group, unilateral Labyrinthectomy(UL) was performed on the right ear under anesthesia via a preauricular incision. The tympanic membrane,malleus and incus were removed. A fine electric cautery tool was used to cauterize the stapedial artery. The stapes was lifted off the oval window to expose the vestibule. The utricle and saccule were carefully excised. The saccule and semicircular canals were destroyed by aspiration. After destruction of all the vestibular end organs was visually confirmed, the vestibule was filled with Gelfoam soaked with absolute ethanol. In the sham surgery group, the external auditory canal was exposed via the same preauricular incision and then the skin was sutured.Mice were observed after the surgery for symptoms and underwent Pole test and Beam-crossing test ten days after surgery. Results Strong head tilt and clearly deviated postures were observed in all mice following UL, with obvious tendency of body roll and circling movement toward the impaired ears, as well as rapid spinning on body axis when picked up by the tail. Circling and body roll resolved gradually in the first week after surgery. Pole test and Beam-crossing test ten days after surgery showed significant differences between the two groups(P=0.000 and 0.002, respectively). Conclusion In the first week after UL, while some vestibular symptoms resolved gradually, vestibular compensation in mice is yet complete ten days after the surgery. With the use of absolute ethanol, UL in mice is an optimal model of acute vestibular injury for its reliability and effectiveness.
作者
王颖怡
解龙昌
陈文勇
陈伟杰
陈盛强
袁忠民
崔勇
WANG Yingyi;XIE Longchang;CHEN Wenyong;CHEN Weijie;CHEN Shengqiang;YUAN Zhongmin;CUI Yong(Department of Otorhinolaryngology,Guangdong Provincial People’s Hospital,Guangzhou,510080,China;Second Affiliated Hospital of Guangzhou Medical University,Guangzhou,510260,China;Department of Otorhinolaryngology,Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,510120,China;Guangzhou University of Chinese Medicine,Guangzhou,510000,China;Guangzhou Medical University,Guangzhou,510000,China)
出处
《中华耳科学杂志》
CSCD
北大核心
2019年第6期963-967,共5页
Chinese Journal of Otology
基金
广东省自然科学基金项目：胚胎干细胞及诱导性多能干细胞向内耳纤维细胞诱导分化研究(编号：2015A030313535)~~
关键词
急性前庭损伤
前庭代偿
小鼠模型
单侧迷路切除术
Acute Vestibular Injury
Vestibular Compensation
Mouse model
Unilateral Labyrinthectomy
