摘要
目的探讨天麻醇提取物对模型小鼠学习、记忆的改善作用。方法将100只小鼠随机分为正常对照组(A组,等体积蒸馏水),模型组(B组,等体积蒸馏水)、吡拉西坦组(C组,700 mg/kg),天麻醇提取物高、低剂量组(D1组、D2组,1.0,0.4 g/kg),灌胃给药,灌胃体积为每10 g体质量0.2 mL,每天1次,连续30 d。灌胃结束第2天开始,B组、C组、D1组、D2组大鼠均先后予以腹腔注射东莨菪碱(1 mg/kg)、氯霉素(200 mg/kg),以及灌胃20%乙醇(每10 g体质量0.2 mL),依次复制小鼠记忆获得、巩固和再现障碍模型,均每天1次,连续6 d,间隔5 d再进行下个建模。A组小鼠腹腔注射等体积0.9%氯化钠注射液和灌胃给予等体积蒸馏水。行为学检测与建模同时开始,测定小鼠120 s内的逃避潜伏期和空间搜索距离,计算滞留时间、搜索距离百分比。结果与A组比较,记忆获得障碍模型组、记忆巩固障碍模型组小鼠逃避潜伏期和搜索距离均显著延长,滞留时间百分比和搜索距离百分比均显著降低,记忆再现障碍模型组小鼠逃避潜伏期显著延长(P<0.01或P<0.05)。与记忆获得障碍模型组比较,C组、D1组和D2组小鼠的逃避潜伏期和搜索距离均显著缩短(P<0.01或P<0.05);与记忆巩固障碍模型组比较,C组、D1组和D2组小鼠的逃避潜伏期和搜索距离均显著缩短,C组和D1组小鼠的滞留时间百分比和搜索距离百分比均显著升高(P<0.01或P<0.05);与记忆再现障碍模型组比较,C组和D1组小鼠的逃避潜伏期显著缩短(P<0.01)。结论天麻醇提取物对模型小鼠学习、记忆有一定改善作用。
Objective To investigate the improvement effect of alcohol extract of Gastrodiae Rhizoma on learning and memory of model mice.Methods Totally 100 mice were randomly divided into the normal control group(group A,equal volume of distilled water),model group(group B,equal volume of distilled water),piracetam group(group C,700 mg/kg),alcohol extract of Gastrodiae Rhizoma high dosage group and low dosage group(group D1 and group D2,1.0 and 0.4 g/kg),all mice were administrated by gavage,the volume was 0.2 mL/10 g of body weight,once a day for 30 d.From the second day after administration,the mice in group B,group C,group D1 and group D2 were given intraperitoneal injection of scopolamine(1 mg/kg),chloramphenicol(200 mg/kg),and given intragastric administration of 20%ethanol(0.2 mL/10 g of body weight),and then the memory acquisition impairment model,memory consolidation impairment model,memory reappearance impairment model were reproduced,once a day for 6 d,and then the next models were established at 5 d intervals.The mice in group A were given intraperitoneal injection of 0.9%Sodium Chloride Injection and intragastric administration of equal volume of distilled water.At the same time,the behavioral detection and modeling were started.Escape latency and spatial search distance were measured within 120 s,the retention time and percentage of search distance were calculated.Results Compared with those in group A,the escape latency and search distance of rice were significantly prolonged,while the retention time and percentage of search distance were significantly reduced in the memory acquisition impairment model group and memory consolidation impairment model group,and the escape latency of mice in the memory reappearance impairment model group was significantly prolonged(P<0.01 or P<0.05).Compared with those in the memory consolidation impairment model group,the escape latency and search distance of the mice in group C,group D1 and group D2 were significantly shortened(P<0.01 or P<0.05).Compared with the memory consol
作者
冯晋
何芳雁
夏霜莉
郑秋合
代蓉
FENG Jin;HE Fangyan;XIA Shuangli;ZHENG Qiuhe;DAI Rong(School of Traditional Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming,Yunnan,China 650000)
出处
《中国药业》
CAS
2019年第22期16-18,共3页
China Pharmaceuticals
基金
云南省科技厅科技计划项目[2018FF001(-007),2017FF117(-004),2018FF001(-074)]
关键词
天麻醇提取物
东莨菪碱
氯霉素
乙醇
改善记忆
MORRIS水迷宫
ethanol extract of Gastrodiae Rhizoma
scopolamine
chloramphenicol
ethanol
memory improvement
Morris water maze
