摘要
目的探讨儿童急性淋巴细胞白血病(ALL)患者骨髓中miRNA-326的表达及其临床意义。研究N-ras基因在儿童ALL微小残留病(MRD)中的表达,以期为儿童ALL的复发提供生物学检测标志。方法本研究选取35例儿童ALL和35例非白血病儿童作为对照组,通过实时荧光定量PCR(RT-PCR)检测miRNA-326在儿童ALL中相对表达量,使用单链构象多态性PCR(PCR-SSCP)检测N-ras基因在治疗1年后的微小残留病(MRD)中的变化。结果miRNA-326在儿童ALL中的相对表达量(2.22±0.72)较对照组(6.35±1.23)明显下降;SSCP的电泳结果显示MRD组患者N-ras基因的电泳速度较对照组快,基因突变率为44.44%。结论miRNA-326和N-ras基因的检测可作为儿童ALL的诊断及复发生物学标志,也可为白血病的耐药性监测和治疗提供新的靶点。
Objective To investigate the expression and clinical significance of miRNA-326 in bone marrow of pediatric acute lymphoblastic leukemia(ALL).And to study the expression of N-ras gene in ALL minimal residual disease(MRD),which provide biological markers for the recurrence of ALL.Methods A total of 35 children with ALL and 35 children with non-leukemia were selected as control group.Real-time fluorescence quantitative PCR(RT-PCR)was used to detect the relative expression of microRNA-326 in children with ALL,and single strand conformation polymorphism PCR(PCR-SSCP)was used to detect the changes of N-ras gene in MRD after 1 year treatment.Results The relative expression of miRNA-326 in pediatric ALL(2.22+0.72)was significantly lower than that of control group(6.35+1.23).The electrophoresis results of SSCP showed that the electrophoresis speed of N-ras gene in MRD group was faster than that of control group.The mutation rate was 44.44%.Conclusion The detection of miRNA-326 and N-ras genes can be used as biological markers for the diagnosis and recurrence of pediatric ALL,which can also provide new targets for drug resistance monitoring and treatment of leukemia.
作者
王利霞
李素颜
Wanglixia;Lisuyan(Department of Clinical Laboratory,Anyang People's Hospital,Anyang,455000,Henan province,China;Department of Hematology,Anyang People's Hospital,Anyang,455000 Henan,province,China)
出处
《实验与检验医学》
CAS
2019年第6期1009-1011,1021,共4页
Experimental and Laboratory Medicine
基金
2018年河南省医学科技攻关项目，2018020613
