摘要
为了对黑龙江省规模化奶牛场牛病毒性腹泻/黏膜病进行检疫净化,试验应用qRT-PCR法检测了黑龙江省4个规模化奶牛场(A^D场)奶牛耳组织样品(共1286份,58组),qRT-PCR法检测得到的阳性组样品再用双抗夹心ELISA进行每份样品的检测。阳性牛间隔1个月用RT-PCR方法复检。结果表明:A场牛病毒性腹泻病毒阳性率为0.5%(1/193),B场为0.1%(2/875),C场为1.8%(1/55),D场未检测出牛病毒性腹泻/黏膜病抗原阳性牛(0/163)。间隔1个月,用RT-PCR方法复检,阳性符合率100%。说明qRT-PCR结合双抗夹心ELISA方法适合于规模化奶牛场牛病毒性腹泻/黏膜病的快速、特异性的检疫净化。
To quarantine porification the infection of bovine viral diarrhea-mucosal disease(BVD-MD)in large-scale cattle farms in Heilongjiang Province,ear tissue samples of dairy cattle form(a total of 1286,58 groups)four large-scale dairy farms(named A,B,C,D)in Heilongjiang Province were tested by qRT-PCR and double-antibody sandwich ELISA.The positive cattle were rechecked by PCR one month later.The results showed that the Bovine viral diarrhea virus(BVDV)-positive cow was detected in 193 cattle in the farm A,with a positive rate of 0.5%(1/193);two BVDV positive cattle were detected in the farm B,the positive rate was 0.1%(2/875);one BVDV positive cow was detected in the farm C,the positive rate was 1.8%(1/55);no BVDV antigen-positive cattle were detected in the farm D(0/163).After one month interval,the positive coincidence rate was 100%retested by RT-PCR method.It indicates that qRT-PCR combined with double-antibody sandwich ELISA are rapid and specific and suitable for quarantine and removal of BVDV in large-scale dairy farm.
作者
张世勋
赫鸣睿
于洪江
何泊宁
赵尚琪
王丽
吴陈华
黄雯静
王天
刘宇
岳山
刘珊珊
翟军军
宋佰芬
朱战波
ZHANG Shixun;HE Mingrui;YU Hongjiang;HE Boning;ZHAO Shangqi;WANG Li;WU Chenhua;HUANG Wenjing;WANG Tian;LIU Yu;YUE Shan;LIU Shanshan;ZHAI Junjun;SONG Baifen;ZHU Zhanbo(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Heilongjiang Provincial Cattle Disease Prevention and Control Engineering Technology Research Center,Daqing 163319,China;Thermo Fisher Scientific Co.,Ltd.,Shanghai 200000,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2019年第22期72-75,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
“十三五”国家重点研发计划课题项目(2016YFD0500904)
黑龙江省青年科学基金项目(QC2015031)
