摘要
叶片是植物进行光合作用的主要器官,马铃薯叶片的光合能力与植株的生物量以及薯块的产量具有密切的关系.研究构建了马铃薯叶片发育畸形的分离群体,基于高通量测序的混合群体分离分析,将控制马铃薯叶片发育的基因定位于ch02:36.06 Mb-48.24 Mb区间.进一步通过精细定位将候选基因缩小范围至400 kb的区间,最后结合基因组注释信息,筛选出一个与叶绿体发育相关的基因作为候选基因.
Leaf is an important organ for photosynthesis in plant.The photosynthetic capacity of potato leaf was correlated with the potato yield.In this study,we constructed a segregation population with different leaf phenotypes,and mapped the genes controlling potato leaf development to the genomic region,ch02:36.06 Mb-48.24 Mb by high-throughput sequencing based Bulked Segregant Analysis(BSA)-seq.The candidate gene was further narrowed down to a 400 kb interval by molecular marker-based fine mapping,and finally a gene related to chloroplast development was identified as a candidate gene aided by the genome annotation information.This study not only provide that how high-throughput sequencing could be used to map the gene,but also laid the foundation for the subsequent gene functional verification,genetic regulation of this trait.
作者
张黎冬
杨中敏
唐蝶
杨典
李晓凤
祝光涛
ZHANG Li-dong;YANG Zhong-min;TANG Die;YANG Dian;LI Xiao-feng;ZHU Guang-tao(School of Life Science,Joint Academy of Potato Science,Key Laboratory for Potato Biology of Yunnan Province,Key Laboratory for Potato Biology in Universities of Yunnan Province,Yunnan Normal University,Kunming 650500,China)
出处
《云南师范大学学报（自然科学版）》
2019年第6期53-59,共7页
Journal of Yunnan Normal University:Natural Sciences Edition
基金
云南省高端人才引进计划资助项目(2013HA025)
关键词
马铃薯
叶片发育
遗传分析
Potato
Leaf development
Genetic analysis
