摘要
目的探讨标本温度、存储时间对肝功能生化检验结果的影响,为提高检验质量提供重要依据。方法选取2018年10月~2019年2月我院检验科接受肝功能生化检验的200例健康者作为研究对象,均采集晨起空腹外周静脉血3.0 ml/管,共6管,比较常温及4℃环境,即刻及存储12 h检验,溶血及无溶血标本中肝功能生化指标水平。结果无溶血标本的DBIL、AST、ALT、ALB水平高于溶血标本,ALP、TP、TBIL水平低于溶血标本,差异有统计学意义(P<0.05)。常温与4℃保存标本的DBIL、TBIL、AST、ALT、ALB、ALP、TP水平比较,差异无统计学意义(P>0.05)。即刻与存储12 h标本的DBIL、TBIL、AST、ALT、ALB、ALP、TP水平比较,差异无统计学意义(P>0.05)。结论血样标本保存温度、存储时间对肝功能生化检验结果影响甚微,但是溶血会对检验指标产生一定影响,因而在采血后特别注意标本状态变化,规避溶血现象,以保证检验结果的准确性。
Objective To investigate the effects of specimen temperature and storage time on the biochemical test results of liver function, and to provide an important basis for improving the quality of the test. Methods From October 2018 to February 2019, 200 healthy subjects who underwent biochemical tests of liver function in our laboratory were selected as subjects. They were collected from the morning venous blood of 3.0 ml/tube, a total of 6 tubes, compared with normal temperature and 4℃ environment, immediate and stored 12h test, hemolysis and non-hemolytic specimens in the liver function biochemical indicators.Results The levels of DBIL, AST, ALT and ALB in hemolysis-free specimens were higher than those in hemolysis specimens. The levels of ALP, TP and TBIL were lower than those in hemolysis specimens,the difference was statistically significant( P<0.05). There were no significant differences in the levels of DBIL, TBIL, AST, ALT, ALB, ALP and TP between the samples at room temperature and 4℃(P>0.05). There was no significant difference in DBIL, TBIL, AST, ALT, ALB, ALP, and TP levels between the 12h specimens stored immediately (P >0.05). Conclusion The preservation temperature and storage time of blood sample have little effect on the biochemical test results of liver function, but hemolysis will have certain influence on the test index. Therefore, after blood collection, special attention should be paid to the state change of the specimen to avoid the hemolysis phenomenon to ensure the accuracy of the test result.
作者
黄士昌
HUANG Shi-chang(Department of Clinical Laboratory,Le'an County Traditional Chinese Medicine Hospital,Le'an 344300,Jiangxi,China)
出处
《医学信息》
2019年第22期172-174,共3页
Journal of Medical Information
关键词
血样标本
保存稳温度
存储时间
溶血
肝功能
Blood sample
Storage stable temperature
Storage time
Hemolysis
Liver function
