摘要
目的研究βⅡ-血影蛋白(SPTBN1)在鼻咽癌细胞、组织和血清中的表达水平,并联合早期抗原抗体(EA-IgA)、核抗原抗体(VCA-IgA)研究其在鼻咽癌诊断中的临床价值。方法自2016年12月至2018年12月,培养NP69、6-10B、5-8F细胞系,并收集临床鼻咽癌和非鼻咽癌患者共计71份鼻咽部活检标本、130份血清,通过蛋白印迹实验、免疫组化实验及ELISA实验检测SPTBN1蛋白在鼻咽癌细胞、组织及体内的表达水平与非鼻咽癌组是否有差异,并通过Logistic回归分析建立血清指标预测鼻咽癌的统计学模型。结果NP69、6-10B、5-8F细胞系间SPTBN1蛋白表达水平呈逐步下降趋势(H=50.205,P<0.05);鼻咽癌组织中SPTBN1蛋白表达阳性率(86.7%)较非鼻咽癌组低(43.9%,χ2=13.443,P<0.001);与非鼻咽癌组比较,鼻咽癌组血清中SPTBN1蛋白水平[73(42,142)]较低而EA-IgA、VCA-IgA水平[3.42(1.29,5.19)、3.55(1.95,5.01)]较高(HSPTBN1=24.105,HEA-IgA=52.398,HVCA-IgA=70.426,P<0.001)。联合预测鼻咽癌患病模型预测一致率为96.2%。受试者工作特征曲线评估SPTBN1蛋白单独及联合EA-IgA、VCA-IgA诊断鼻咽癌的曲线下面积分别为0.794与0.987(Z=4.900,P<0.001)。结论SPTBN1蛋白在鼻咽癌细胞、组织和血清中的表达均较正常水平低,有望成为鼻咽癌诊断的新指标。SPTBN1与EA-IgA、VCA-IgA联合检测鼻咽癌具有较好的敏感度和特异度。
Objective The expression of spectrin beta chain,brain1(SPTBN1)were measured in nasopharyngeal carcinoma(NPC)cell lines,as well as tissues and serums of NPC cases and normal controls.The clinical value of SPTBN1 expression for NPC diagnosis were assessed along with the antibody levels of early antigen-IgA(EA-IgA)and viral capsid antigen-IgA(VCA-IgA).Methods A total of 71 nasopharynx tissue specimens and 130 serum from both NPC cases and matched health controls were collected from December 2016 to December 2018.In logistic regression the levels of SPTBN1,EA-IgA,VCA-IgA were identified and included in an integrative risk prediction model.Discriminatory accuracy was measured by generation of receiver operator curves and estimation of area under the curve(AUC).Results The SPTBN1 concentration in cell lines from NP69,6-10B to 5-8F showed a decreasing trend(H=50.205,P<0.05).The SPTBN1 expression level in serums were significantly lower[73(42,142)]compared to controls,while EA-IgA and VCA-IgA levels[3.42(1.29,5.19),3.55(1.95,5.01)]were higher in NPC serum samples(HSPTBN1=24.105,HEA-IgA=52.398,HVCA-IgA=70.426,P<0.01).The findings were confirmed in tissues that the positive SPTBN1 rate were significantly lower in cases(86.7%)as compared to controls(43.9%,χ2=13.443,P<0.001).In the risk prediction model for NPC diagnosis,the AUC of SPTBN1 alone and the integrated regression model were 0.794 and 0.987,respectively(Z=4.900,P<0.01).Conclusions The lower expression of SPTBN1 was a potential marker in NPC diagnosis.The combined analysis of SPTBN1,EA-IgA and VCA-IgA may improve the sensitivity and specificity in NPC diagnosis.
作者
谢旭琳
梁运来
向满林
任煜培
马雅婷
易斌
Xie Xulin;Liang Yunlai;Xiang Manlin;Ren Yupei;Ma Yating;Yi Bin(Department of Clinical Laboratory,Xiangya Hospital,Central South University,Changsha 410008,China)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2019年第10期869-874,共6页
Chinese Journal of Laboratory Medicine
基金
中南大学2017年研究生自主探索创新项目(2017zzts894)。
