摘要
金荞麦作为蓼科多年生草本植物,因其根茎富含大量黄酮类物质而具有极高的药用价值。野生金荞麦在我国分布极其广泛,尤其是云贵川等西南地区。野生荞麦资源考察组在云南进行历时20 d的考察,在丽江、大理、昆明等地采集到14份不同地区的野生金荞麦资源。考察过程中发现,野生金荞麦在植株及果实形态上均存在一定的差异,与其所处的地理环境、海拔、气候条件相关。利用分光光度法和高效液相色谱(HPLC)分别测量采集的块状茎中的总黄酮及黄酮类物质芦丁、槲皮素含量,发现不同地区的野生金荞麦块状茎之间黄酮类物质的含量存在着很大的差异。使用ITS、matK分子标记手段,进行遗传多样性和亲缘关系分析,发现其分类与地区海拔具有较为明显的关系。
Fagopyrum cymosum(Trevir.)Meisn.is a perennial herb of the family Polygonaceae,which has a very high medicinal value because its swollen stems are rich in flavonoids.Wild Fagopyrum cymosum(Trevir.)Meisn.is widely distributed in China,especially in southwestern areas such as Yunnan,Guizhou,Sichuan.Our research group conducted a 20-day study in Yunnan and collected wild Fagopyrum cymosum(Trevir.)Meisn.resources from 14 different regions in Lijiang,Dali and Kunming.There were some differences in the plant or fruit morphology of wild golden buckwheat in different regions,which may be related to the geographical environment and climatic conditions.The contents of total flavonoids,rutin and quercetin in the swollen stems of wild golden buckwheat were measured by spectrophotometry and high performance liquid chromatography(HPLC).Results showed that the flavonoid contents of the swollen stems from different regions were remarkably different.Using ITS and matK molecular markers,genetic diversity and genetic relationship analysis were carried out,and it was found that the classification had a significant relationship with regional altitude.
作者
程成
张凯旋
唐宇
邵继荣
严明理
周美亮
CHENG Cheng;ZHANG Kai-xuan;TANG Yu;SHAO Ji-rong;YAN Ming-li;ZHOU Mei-liang(College of Life Science,Hunan University of Science and Technology,Xiangtan 411201;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;College of Food Science and Technology,Sichuan Tourism University,Chengdu 610100;College of Life Science,Sichuan Agricultural University,Yaan 625001)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第6期1438-1446,共9页
Journal of Plant Genetic Resources
基金
国家重点研发计划中欧政府间合作项目(2017YFE0117600)
国家自然科学基金面上项目(31871536)
中国科协中国作物学会青年托举计划(2017QNRC182)~~
关键词
金荞麦
资源考察
云南
黄酮类
遗传多样性
Fagopyrum cymosum
germplasm investigation
Yunnan
flavonoids
genetic diversity
