摘要
目的:探索注射用黄芪多糖促进人淋巴细胞体外增殖及对于人未分化胃癌细胞HGC-27体外杀伤作用。方法:取正常健康人全血10ml,分离获得原代人淋巴细胞并进行体外培养。利用不同浓度的黄芪多糖进行干预,CCK-8法检测细胞增殖、Elisa法检测IFN-γ的表达。以人未分化胃癌细胞HGC-27为靶细胞进行体外杀伤实验,CCK-8检测肿瘤细胞存活率。结果:黄芪多糖对人淋巴细胞体外有增殖有一定的促进作用,体外有明显的肿瘤细胞杀伤作用,相关的肿瘤细胞杀伤作用可能与促进IFN-γ的分泌有关。结论:黄芪多糖可以增强人免疫系统功能,且可以促进淋巴细胞的增殖,IFN-γ的分泌。这些功能可能是肿瘤细胞毒性的药理基础。
Objective:To study the effect of Astragalus Polysaccharide Injection promoted human lymphocyte proliferation and for HGC-27 in vitro cytotoxicity. Methods:Useing normal healthy human whole blood 10 ml, primary human lymphocyte isolated and cultured in vitro. APS intervention in different concentrations, CCK-8 assay cell proliferation, expression of Elisa assay of IFN-γ. HGC-27 to the tumor target cells, CCK-8 detection Astragalus Polysacharin by stimulating lymphocytes cytotoxicity of the HGC-27. Results:Astragalus Polysacharin had effect of promoting the proliferation of lymphocytes in a dose-dependent manner and can promote lymphocytes cell activation to kill tumer cells in vitro. Associated with the tumor cell killing effects may promote the secretion of IFN-γ related. Conclusion:Astragalus Polysaccharide can enhance the human immune system function, promotion the proliferation of lymphocytes, secretion of IFN-γ. These features may be the pharmacological basis of tumor cytotoxic.
作者
廖大忠
LIAO Dazhong(Department of Oncology,the Affiliated Traditional Chinese Medicine Hospital of Luzhou Medical College(Luzhou Sichuan 646000,China)
出处
《四川中医》
2019年第11期58-60,共3页
Journal of Sichuan of Traditional Chinese Medicine
