摘要
目的研究干扰素体外释放酶联免疫检验技术在结核病诊断中的应用价值。方法取我院收治的结核病患者120例为研究组,根据结核类型划分为肺结核与肺外结核各60例,另择取同期健康体检者(排除肺结核意外的肺部疾病患者)60例作为对照组,所有研究对象均采用结核抗体检验、皮肤过敏实验与干扰素体外释放酶联免疫检验,比较两组三项检测技术的阳性率。结果与对照组比较,结核病组三项检测技术的阳性率均高,与结核菌素皮肤过敏实验比较,干扰素体外释放酶联免疫检验诊断肺结核与肺外结核患者具有较高的阳性率,P <0.05(具统计学差异)。结论对结核病患者采用干扰素体外释放酶联免疫检验诊断能明确机体是否感染结核病杆菌,采用试剂盒以抗原特异性为基点对T细胞产生刺激,产生免疫应答提高检测可靠性,在体外检测中被广泛应用,便于为患者后期临床治疗提供重要参考,值得借鉴。
Objective To study application value of interferon in vitro release enzyme-linked immunoassay(ELISA) in diagnosis of tuberculosis. Methods Choose 120 cases tuberculosis patients admitted to our hospital as research group. According to tuberculosis type, divide them into 60 cases of tuberculosis and 60 cases of extrapulmonary tuberculosis. Choose another 60 cases healthy people(with pulmonary diseases excluding pulmonary tuberculosis) as control group. Carry on tuberculosis antibody test, skin allergy test and interferon release enzymelinked immunoassay in vitro for all cases. Compare positive rate of three detection techniques of two groups. Results Compared with control group, positive rates of three detection techniques in tuberculosis group were higher. Compared with skin allergy test of tuberculin, IFN-ELISA has higher positive rates in diagnosis of tuberculosis and extrapulmonary tuberculosis were(P< 0.05), with statistical significance. Conclusion Interferon in vitro release of enzyme-linked immunoassay diagnosis can confirm if body infected with tuberculosis bacilli of tuberculosis patients. Stimulating T cells based on antigen specificity with kit, can generate immune response and improve reliability of detection, which is widely applied for in vitro detection, to provide important reference for later clinical treatment of patients.
作者
蔡国芳
CAO Guo-fang(Zhangzhou City Zhangpu County Hospital of Fujian,Zhangzhou,Fujian,363200)
出处
《智慧健康》
2019年第30期80-81,共2页
Smart Healthcare
关键词
干扰素体外释放酶联免疫检验技术
结核病
诊断价值
In vitro release enzyme-linked immunoassay of interferon
Tuberculosis
Diagnostic value
