摘要
目的探讨三芪口服液治疗糖尿病肾病的作用机制。方法根据药物的ADME特性,利用TCMSP平台筛选以三七、黄芪复方研制的三芪口服液的活性成分与作用靶点,构建成分靶点网络;基于GeneCards数据库、DisGeNET数据库、OMIM数据库检索糖尿病肾病相关靶点;采用Cytoscape软件构建活性药物-活性成分-基因靶点,并将获得的靶点信息利用DAVID数据库分析生物过程及通路富集。结果通过筛选得到27个药物活性成分,共涉及191个作用靶点,与疾病靶点有关的潜在靶点为118个,主要通过调控PPARG、IL-6等靶蛋白及AGE-RAGE信号通路、流体剪切应力和动脉粥样硬化、IL-17信号通路、HIF-1信号通路等发挥糖尿病肾病的治疗作用。结论三芪口服液通过多靶点-多通路治疗糖尿病肾病,为解释其治疗作用机制提供理论基础及科学依据。
Objective To explore the mechanism of Sanqi Oral Liquid in the treatment of diabetic nephropathy.Methods According to the ADME characteristics of the drug,TCMSP platform was used to screen the active ingredients and targets of Sanqi Oral Liquid,which was developed with the compound of Sanqi and Huangqi,and the component target network was constructed.Retrieval of diabetic nephropathy related targets based on GeneCards database,DisGeNET database and OMIM database.The target of active drugs,active ingredients and genes was constructed by using the software of Cytoscape,and the target information was analyzed by using the database of DAVID and the biological process and pathway enrichment were analyzed.Results There were 27 active components were screened,involving 191 targets,and 118 potential targets related to disease targets.The therapeutic effect of diabetic nephropathy was mainly achieved by regulating PPARG,IL-6 and age-range signaling pathway,fluid shear stress and atherosclerosis,IL-17 signaling pathway,HIF-1 signaling pathway,etc.Conclusion Sanqi Oral Liquid can treat diabetic nephropathy through multi-target and multi-channel,which provides theoretical basis and scientific basis for explaining its therapeutic mechanism.
作者
林嘉荣
张蕾
桂定坤
曾贵兴
吴禹池
邹川
林启展
LIN Jiarong;ZHANG Lei;GUI Dingkun;ZENG Guixing;WU Yuchi;ZOU Chuan;LIN Qizhan(The Second Clinical Medical College,Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou510405,China;Department of Nephrology,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou510120,China;Department of Nephrology,the Sixth People′s Hospital Affiliated to Shanghai Jiaotong University,Shanghai200233,China)
出处
《中国医药导报》
CAS
2019年第30期130-135,F0004,共7页
China Medical Herald
基金
国家自然科学基金资助项目（81603717、81573738）
广东省中医院张琪学术经验传承工作室（E43712）
关键词
三芪口服液
糖尿病肾病
网络药理学
作用机制
Sanqi Oral Liquid
Diabetic nephropathy
Network pharmacology
Mechanism of action
