摘要
目的观察慢性间歇缺氧(CIH)小鼠一氧化氮(NO)水平的变化趋势.方法将12只11周龄雄性小鼠随机等分为两组,CIH组以间歇缺氧饲养,对照组(UC)以常氧饲养,分别于第2、6、9周抽血,采用ELISA法检测小鼠NO水平.结果①CIH组与UC组比较:第2、6周CIH组小鼠NO水平均明显低于UC组[分别为(1.3±0.59)μmol/L比(3.34±0.92)μmol/L,(3.9±0.83)μmol/L比(7.11±1.68)μmol/L],差异有统计学意义(t=3.67,P<0.05;t=3.34,P<0.05);第9周CIH组小鼠NO水平低于UC组[分别为(1.76±0.72)μmol/L比(2.45±0.77)μmol/L],但未见统计学差异(t=l.31,P>0.05).②CIH组间比较:第6周NO水平明显高于第2周,差异有统计学意义(P<0.01);第9周NO水平明显低于第6周,差异有统计学意义(P<0.01).③UC组比较:第6周NO水平明显高于第2周,差异有统计学意义(P<0.01);第9周NO水平明显低于第6周,差异有统计学意义(P<0.01).结论①间歇缺氧过程中NO水平明显均低于对照组,至血管内皮保护功能下降;②间歇缺氧第2周出现NO水平降低,早期即可导致血管内皮保护功能下降;③两组第6周小鼠血清NO水平均明显高于第2周,第9周小鼠血清NO水平均明显低于第6周,可能衰老及炎症反应[诱导性一氧化氮合酶(iNOS)]参与调控血管内皮的保护作用;④CIH组第6周NO水平代偿性升高,第9周失代偿下降,建议OSA治疗时机选择最好在间歇缺氧6周前.
Obiective To observe the trend of nitric oxide(NO)levels in mice with chronic intermittent hypoxia(CIH).Methods Twelve 11-week-old male mice were randomly divided into two groups.The CIH group was fed with intermittent hypoxia and the control group(UC)was fed with normoxia.Blood was drawn at week 2,6 and 9,respectively 11-week-old male mice were randomly divided into two groups.CIH group was fed with intermittent hypoxia(8 h/d)and control(UC)group was fed with normoxic feeding.Blood samples were taken from the two groups at the 2nd,6th and 9th week,respectively and the NO level of mice was detected by ELISA method.Results⑴Compared with UC group,the level of NO in CIH group was significantly lower than that in UC group at the 2nd and 6th week[(1.3±0.59)umol/L vs.(3.34±0.92)umol/L,(3.9±0.83)umol/L vs.(7.11±1.68)umol/L](t=3.67,P<0.05;t=3.34,P<0.05),the level of NO in CIH group was lower than that in UC group at the 9th week[(1.76±0.72)umol/L vs.(2.45±0.77)umol/L](t=1.31,P>0.05).⑵Comparison between CIH groups:the level of NO in the 6th week was significantly higher than that in the 2nd week(P<0.01)and the level of NO in the 9th week was significantly lower than that in the 6th week(P<0.01).⑶In UC group,the level of NO in the 6th week was significantly higher than that in the 2nd week(P<0.01)and the level of NO in the 9th week was significantly lower than that in the 6th week(P<0.01).Conclusion⑴The level of serum NO in intermittent hypoxia group was lower than that in normoxic group,which resulted in the decrease of vascular endothelial protective function.⑵The level of serum NO decreased in the 2nd week of intermittent hypoxia,which led to the decrease of vascular endothelial protective function in the early stage.⑶The level of serum NO in two groups at the 6th week was significantly higher than that at the 2nd and the level of serum NO at the 9th was significantly lower than that at the 6th.Senescence and inflammation[inducible nitric oxide synthase(iNOS)]may be involved in the regulation of vascul
作者
路申潞
王瑞英
牛雅琼
彭智柯
熊上
郑晓斌
葛婕丽
罗宏
LU Shen-lu;WANG Rui-ying;NIU Ya-qiong;PENG Zhi-ke;XIONG Shang;ZHENG Xiao-bin;GE Jie-li;LUO Hong(The Second Clinical Medicine College,Shanxi Medical University,Taiyuan 030000,China;Department of Cardiology,Affiliated Hospital of Shanxi Medical University,Taiyuan 030000,China;Department of Neurology,The Eighth People's Hospital of Taiyuan,Taiyuan 030000,China;Department of Respiratory Medicine,Association of Taiyuan Medicine,Taiyuan 030000,China)
出处
《中国心血管病研究》
CAS
2019年第11期1028-1031,共4页
Chinese Journal of Cardiovascular Research
