摘要
目的探讨色素上皮衍生因子(PEDF)对亚砷酸钠诱导的血管内皮细胞功能失调的作用。方法以人脐静脉内皮细胞系EA.hy926作为研究对象,设置0(对照)、1、2、5、10、20、50μmol/L亚砷酸钠组,处理细胞24h,CCK8法检测细胞活力,比色法检测细胞培养物上清诱导型一氧化氮合酶(iNOS)活性,酶联免疫吸附实验(ELISA)检测细胞培养物上清中PEDF含量,流式细胞术检测细胞内一氧化氮(NO)含量。结果20、50μmol/L组细胞活力[(80.69±7.95)%、(69.87±10.54)%]明显低于对照组[(101.08±3.22)%,P均<0.05]。10、20、50μmol/L组细胞培养物上清中iNOS活性、PEDF含量[(829.1±68.2)、(772.3±37.1)、(874.6±43.5)U/L,(12.06±0.55)、(11.97±0.39)、(13.89±0.26)mg/L]均高于对照组[(397.5±43.5)U/L,(10.70±0.35)mg/L,P均<0.01],50μmol/L组细胞培养物上清中PEDF含量最高。50μmol/L组细胞内NO含量(11558.99±397.43)明显低于对照组(14131.49±262.61,P<0.01)。结论PEDF参与砷诱导的血管内皮细胞功能失调。
Objective To explore the role of pigment epithelium-derived factor (PEDF) in vascular endothelial cells dysfunction induced by sodium arsenite (NaAsO2). Methods Human umbilical vein endothelial cells (EA.Hy926 cells) were treated with different levels of NaAsO2 [0 (control), 1, 2, 5, 10, 20, 50 μmol/L] for 24 hours. The cell viability was determined using CCK8. Colorimetric assay was used to detect the activity of inducible nitric oxide synthase (iNOS) in culture supernatants, PEDF content in the supernatant of EA.Hy926 cells was detected by enzyme-linked immunosorbent assay (ELISA), and nitric oxide (NO) content in cells was detected by flow cytometry. Results Compared with the control group[(101.08 ± 3.22)%], the cell viability of 20 μmol/L group [(80.69 ± 7.95)%] and 50 μmol/L group[(69.87 ± 10.54)%] decreased significantly, and the differences were statistically significant (P < 0.05). The activity of iNOS increased significantly in 10, 20, 50 μmol/L groups [(829.1 ± 68.2), (772.3 ± 37.1), (874.6 ± 43.5) U/L], respectively, in comparison with that of the control group [(397.5 ± 43.5) U/L] in the cell culture supernatant (P < 0.01). Similarly, PEDF levels in the groups of 10, 20, 50 μmol/L [(12.06 ± 0.55), (11.97 ± 0.39) and (13.89 ± 0.26) mg/L, respectively] were higher than that of the control group [(10.70 ± 0.35) mg/L, P < 0.01], and the highest content of PEDF was found in 50 μmol/L group. The NO level in 50 μmol/L group (11 558.99 ± 397.43) was significantly lower than that of the control group (14 131.49 ± 262.61, P < 0.01). Conclusion PEDF is involved in the vascular endothelial cells dysfunction induced by arsenic.
作者
张微
赵丽军
李德安
郭相南
王敬丘
Zhang Wei;Zhao Lijun;Li Dean;Guo Xiangnan;Wang Jingqiu(Institute for Iodine Deficiency Disorders Control,Center for Endemic Disease Control,Chinese Center for Disease Control and Prevention,Harbin Medical University,Harbin 150081,China;Institute for Endemic Fluorosis Control, Center for Endemic Disease Control,Chinese Center for Disease Control and Prevention,Harbin Medical University, Harbin 150081,China;Institute of Kaschin-Beck Disease,Center for Endemic Disease Control,Chinese Center for Disease Control and Prevention,Harbin Medical University,Harbin 150081,China)
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2019年第2期107-110,共4页
Chinese Journal of Endemiology
基金
黑龙江省教育厅项目(12541417).
