摘要
植物次生代谢产物是植物长期与生态环境适应的结果,具有多种药理、生理和生态学功能,已广泛应用于医药、保健品、化妆品、食品和农药等行业。文章重点介绍了病原菌、诱导子和内生菌介导植物次生代谢产物累积的概况,指出利用病原菌介导植物次生代谢累积作用不持久和不安全,诱导子介导虽安全但更不持久,内生菌介导远比生物侵害因子更安全、更可控和更持久,且经济可靠效率高,应用前景更理想;同时总结了内生菌介导植物次生代谢产物累积的主要途径,归纳了内生菌与宿主植物次生代谢的互作机制,并指出内生菌在药用植物药效成分累积中的作用机制。今后应借助高通量测序、SSH、HPLC、TLC、IR、MS和NMR等手段确定内生菌介导药用植物次生代谢产物累积的途径、种类及其差异基因,再结合药用植物次生代谢单体和差异基因表达量相关分析、Unigene注释、差异基因结构分析筛选候选基因并验证其功能,综合Unigene蛋白互作网络等分析和关键酶基因作用信号通路,确定内生菌介导药用植物次生代谢累积的分子机制。
Plant secondary metabolites are the result of long-term adaptation of plants to the ecological environment,they have a variety of pharmacological,physiological and ecological functions and are widely used in medicine,health care products,cosmetics,food and pesticide industries.The accumulation of plant secondary metabolites mediated by microorganisms was reviewed in this article,focusing on the pathogens,elicitors,endophytes to mediate plant secondary metabolism:pathogens were not persistent and unsafe.Elicitors were safe but less persistent.Endophytes were far safer,more controllable and lasting,and economically reliable and more efficient than biological aggressor.The main pathways of secondary metabolism mediated by endophytes,and the interaction mechanism between endophytes and host plants were summarized.The function mechanism of endophytes in effective component accumulation of medicinal plants was pointed out.High-throughput sequencing,SSH,HPLC,TLC,IR,MS and NMR were used to determine the pathways and species and differential genes of endophytes mediated medicinal plants secondary metabolism accumulation.In combination with the secondary metabolites of medicinal plants and differential gene expression analysis,Unigene annotation and differential gene structure analysis,candidate genes were screened and their functions were verified.The molecular mechanism of endophytes mediated the accumulation of secondary metabolism in medicinal plants were determined combining with the Unigene protein interaction network and key enzyme gene signaling pathways.
作者
郜玉钢
莫琪琪
赵岩
臧埔
GAO Yu-gang;MO Qi-qi;ZHAO Yan;ZANG Pu(College of Traditional Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Ginseng Engineering Technology Research Center of Jilin Province,Changchun 130118,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2019年第10期2234-2240,共7页
Journal of Southern Agriculture
基金
国家重点研发计划项目（2016YFC0500300）
吉林省科技发展计划项目（20170204017YY,20190301079NY）
关键词
微生物
植物
药用植物
次生代谢产物
互作机制
microbe
plant
medicinal plants
secondary metabolic products
interaction mechanism
