摘要
目的探究梅毒螺旋体酶联免疫吸附实验(TP-ELISA)弱阳性样本与梅毒螺旋抗体明胶颗粒凝集试验(TPPA)检测结果的差异。方法选取2017年12月至2019年3月揭阳市人民医院收治的经TP-ELISA法检测为弱阳性(0.8≤S/CO值≤5)的血液标本作为研究样本,进行TPPA法检测,统计总体诊断符合度,并比较不同S/CO值标本检测符合度差异。结果TP-ELISA检测总体符合率为32.73%(54/165);4.0<S/CO≤5.0组、3.0<S/CO≤4.0、2.0<S/CO≤3.0组组受试者诊断符合率明显高于1.0<S/CO≤2.0组及0.8≤S/CO≤1.0组(P<0.05)。结论梅毒TP-ELISA假阳性高,S/CO值较低的标本诊断符合度较低,通过TPPA的验证可以提高检测结果的准确性。
Objective Investigate the differences between weakly positive specimens of enzyme-linked immunosorbent assay(TP-ELISA)and test results of treponemal pallidum particle agglutination(TPPA).Methods The blood samples of Jieyang People's Hospital from December 2017 to March 2019,which were weakly positive(0.8≤S/CO≤5)by TP-ELISA method,were selected as research samples.TPPA method was used to detect the overall diagnostic coincidence,and the difference of coincidence between different S/CO values was compared.Results The overall coincidence rate of TP-ELISA detection was 32.73%(54/165).The diagnostic coincidence rates in 4.0<S/CO≤5.0 group,3.0<S/CO≤4.0 group and 2.0<S/CO≤3.0 group were significantly higher than those in 1.0<S/CO≤2.0 group and 0.8≤S/CO≤1.0 group(P<0.05).Conclusion The false positives of syphilis TP-ELISA is relatively higher.The diagnostic coincidence degree is lower in specimens with lower S/CO value.Verification by TPPA can improve accuracy of test results.
作者
洪洁玲
HONG Jieling(Jieyang People's Hospital,Jieyang,Guangdong 522000,China)
出处
《大医生》
2019年第12期82-83,共2页
Doctor
关键词
梅毒螺旋体酶联免疫吸附实验
梅毒螺旋抗体明胶颗粒凝集试验
梅毒
弱阳性
treponema pallidum enzyme-linked immunosorbent assay
treponemal pallidum particle agglutination
syphilis
weakly positive
