摘要
利用响应面分析方法对螺旋藻细胞的酶解破壁工艺进行优化。以破壁率为指标,通过单因素试验分别考察料液比、加酶量、酶解温度、时间对螺旋藻破壁的影响。采用单因素试验和Box-Behnken中心组合试验原理确定酶解破壁条件,结果表明,螺旋藻细胞破壁的最佳工艺参数为:料液比1︰200(g/mL)、加酶量2%、酶解温度50℃、酶解时间5 h。在此条件下,螺旋藻细胞的破壁率为85.2%,与预测值84%接近。
Enzymatic hydrolysis was used to break Spriulina cell wall.The effects of solid-liquid radio,enzyme amount,temperature and hydrolysis time on the disruption were investigated by single factor tests and response surface methodology.The experiment results showed that the optimal condition for hydrolysis was:solid-to-liquid ratio of 1︰200(g/m L),enzyme amount of 2%,temperature of 50℃and process time of 5 h.The cell breaking rate was 85.2%under this condition,which perfectly matched the predicted value(84%).
作者
郭锁莲
于雪
边新宇
冷晓爱
姜纯雨
李东华
GUO Suolian;YU Xue;BIAN Xinyu;LENG Xiao’ai;JIANG Chunyu;LI Donghua(College of Pharmaceutical and Biological Engineering,Shenyang University of Chemical Technology,Shenyang 110142;School of Life Sciences and Biotechndogy,Shanghai Jiaotong University,Shanghai 200240)
出处
《食品工业》
CAS
北大核心
2019年第10期224-227,共4页
The Food Industry
基金
国家自然科学青年基金项目（No.21506122）
辽宁省博士启动基金（No.201601197）
关键词
螺旋藻
酶解
破壁
响应面
Spirulina
enzymatic hydrolysis
cell-breakage
response surface methodology
