摘要
使用本实验室保存的重组菌株BL21(DE3)(pET-22b-m)经过IPTG诱导,并且经SDS-PAGE验证,在21.6 Ku处有目的蛋白表达;运用乳化-复乳化的方法,制备IHNV-M蛋白微球疫苗。运用灌胃的方法,将制备好的IHNV-M蛋白微球疫苗免疫虹鳟鱼后,经ELISA测定血清中抗体效价。普通虹鳟鱼组和美国金鳟鱼血清中抗体效价分别为1∶128000和1∶25600。
The recombinant strain BL21(DE3)(pET-22b-m)preserved in our laboratory was induced by IPTG,and confirmed by SDS-PAGE;the target protein was expressed at 21.6Ku.IHNV-M protein microsphere vaccine was prepared by emulsification-double emulsion method.The prepared IHNV-M protein microsphere vaccine was used to immunize rainbow trout with the method of intragastric administration,and the antibody titer in serum was determined by ELISA.The antibody titers in the serum of the common rainbow trout group and the American golden trout were 1∶128000 and 1∶25600,respectively.
作者
潘璇
田晟源
田洋
李全振
赵宝华
PAN xuan;TIAN Sheng-yuan;TIAN Yang;LI Quan-zhen;ZHAO Bao-hua(College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China;Hebei Provincial Fisheries Technology Extension Center,Shijiazhuang 050035,China)
基金
河北省现代农业产业技术体系淡水养殖创新团队资助(项目编号:HBCT2018180207)
河北省科技支撑计划:虹鳟鱼健康养殖及全程质量控制技术集成与示范(项目编号:17226701D)
关键词
造血器官坏死病病毒
基质蛋白M
微球疫苗
免疫原性
hematopoietic necrosis virus
matrix protein M
microsphere vaccine
immunogenicity
