摘要
目的 探讨消癌解毒方对H22荷瘤小鼠移植瘤血管生成相关因子及蛋白的影响。方法 建立H22荷瘤小鼠腹水瘤株体内移植肿瘤模型,空白组每日生理盐水灌胃,消癌解毒方低、中、高剂量组每日10,30,90g·kg-1灌胃,顺铂组每日1 g·kg-1腹腔注射给药,连续给药10 d,记录瘤体体积及移植鼠体重。给药结束,取瘤体组织,称重,计算各给药组的肿瘤抑制率;通过肿瘤组织的全基因组表达谱芯片,Elisa、qRT-PCR、Western-blot观察各给药组对移植瘤的血管生成因子及相关基因STAT1、VEGF、MMP2、TGF-β、CXCL2的影响。结果 与空白组比较,顺铂组及消癌解毒方低、中、高剂量组移植瘤小鼠瘤体重量显著降低(P <0.05),VEGF、MMP2、TGF-β、CXCL2细胞因子水平显著降低(P <0.05);qPCR、western-blot实验结果表明,与空白组比较,消癌解毒方各剂量组STAT1基因及蛋白表达能够显著升高,VEGF、MMP2、TGF-β、CXCL2基因、蛋白的表达显著下降,差异具有统计学意义(P <0.05)。结论 消癌解毒方能够有效抑制H22荷瘤小鼠移植瘤生长,其中抑制肿瘤血管新生是其可能的作用机制之一。
Objective To explore the effects of Xiaoai Jiedu Formula on angiogenesis-related factors and proteins of transplantation tumor in H22 tumor-bearing mice.Methods In vivo tumor-transplantation models of ascitic tumor strains were established in H22 tumor-bearing mice.Blank group was given normal saline by gavage on a daily basis.Xiaoai Jiedu Formula(XJF)groups of low,medium and high doses were gven 10,30 and 90 g·kg-1 Xiaoai Jiedu Formula by gavage everyday.Cisplatin group was given 1 g·kg-1 cisplatin per day by intraperitoneal injection.The medication lasted for 10 d,tumor volume and mice weight were recorded.After the medication,tumor tissue was collected,weight was measured,and tumor suppression rate in each medication group was calculated.According to the results of genome-wide expression profile microarray,Elisa,qPCR and western blot from tumor tissue,the effects of XJF on the expression of STAT1、VEGF、MMP2、TGF-β、CXCL2 were observed.Results Compared with the blank group,the weight of mice in the cisplatin group and the XJF groups of low,medium and high doses was significantly decreased(P<0.05),the levels of VEGF,MMP2,TGF-βand CXCL2 in the XJF groups of low,medium and high doses were significantly decreased,with statistically significant difference(P<0.05).Results of qPCR and western-blot showed that compared with the blank group,the expressions of STAT1 genes and proteins in the XJF groups were significantly increased,VEGF,MMP2,TGF-βand CXCL2 were significantly decreased,with statistically significant difference(P<0.05).Conclusion Xiaoai Jiedu Formula can effectively inhibit the growth of transplantation tumor in H22 tumor-bearing mice,in which one possible mechanism is inhibiting tumor angiogenesis.
作者
李黎
吴勉华
周红光
李沐涵
李文婷
季漪
马艳霞
雒慧娟
Li Li;Wu Mianhua;Zhou Hongguang;Li Muhan;Li Wenting;Ji Yi;Ma Yanxia;Luo Huijuan(Oncology Institute,The First School of Clinical Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center for TCM Cancer Prevention and Treatment,Nanjing 210023,China;Basic Medical School,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2019年第7期1378-1383,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会青年基金项目(81503535):基于mTOR信号通路调控缺氧微环境下细胞自噬的消癌解毒方抗肿瘤作用机制研究,负责人:李黎
国家自然科学基金委员会面上项目(81573910):基于癌毒病机理论的消癌解毒方干预炎癌转化的分子机制研究,负责人:程海波
第六批全国老中医药专家学术经验继承工作(国家中医药人教发[2017]29号):第六批全国老中医药专家学术经验继承人,负责人:李黎
关键词
消癌解毒方
H22荷瘤小鼠
血管生成
全基因组表达谱芯片
Xiaoai Jiedu Formula
H22 tumor-bearing mice
Angiogenesis
genome-wide expression profile microarray
