摘要
为研究不同结构乌鳢短肽螯合肽对抗氧化活性的影响,通过对乌鳢短肽螯合肽粒径、Zeta电位、结构和抗氧化特性等分析,探究螯合肽的结构与抗氧化活性的关系。结果表明,螯合钙和螯合铁的粒径显著变小,而螯合锌与短肽粒径相近。螯合肽的Zeta电位的绝对值较短肽高,螯合肽的结构更稳定。螯合肽中亲水性氨基酸和具有抗氧化活性的氨基酸含量增加,螯合铁中抗氧化活性氨基酸含量占总氨基酸含量最高。螯合肽与短肽的特征吸收峰显示不同离子与短肽的羟基和氨基结合程度不同,螯合铁紫外吸收峰波长最长。电镜分析显示离子与短肽存在吸附作用,螯合铁的吸附作用最强。通过DPPH、H2O2、O2^-·显示螯合肽因结构而产生不同的抗氧化活性和还原能力,其中螯合铁的抗氧化活性最强,其它螯合肽都较短肽具有更强抗氧化活性,螯合肽的抗氧化活性与螯合率呈正相关关系。
In order to study the effect of structure of chelated peptides of Channa argus and antioxidant activity,the chelated peptides particle size,zeta potential,structure and antioxidant activity were analyzed.The relationship between structure and antioxidant activity were determined.The results showed that the particle size of calcium chelated peptides and iron chelated peptides were significantly smaller,but zinc chelated peptides were similar to that of the peptide.The absolute value of zeta potential of chelated peptide was higher than that of short peptide,and the structure of chelated peptide was more stable.The contents of hydrophilic amino acids and antioxidant amino acids were increased,and the highest content of antioxidant amino acids was presented in iron chelated peptides.The characteristic absorption peaks of the chelated peptides and the short peptide showed that the binding degree of the different ions to the short peptide and the amino group.Electron microscopic showed that ions and short peptides were adsorbed together,and the adsorption of iron chelated peptides was the strongest.DPPH,H2O2 and O2^-·showed different antioxidant activity and reducing capacity of chelated peptides due to different structures.Antioxidant activity of iron chelated peptides was strongest,and other chelated peptides have stronger antioxidant activity than that of short peptides.Antioxidant activity of chelated peptide and chelated rate have positively correlated relationship.
作者
汪婧瑜
张业辉
张友胜
刘学铭
程镜蓉
陈智毅
王旭苹
黄利华
Wang Jingyu;Zhang Yehui;Zhang Yousheng;Liu Xueming;Cheng Jingrong;Chen Zhiyi;Wang Xuping;Huang Lihua(Sericultural and Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs,Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610;Guangzhou City Vocational College Food Department,Guangzhou 510405)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2019年第10期93-99,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
广东省自然科学基金重点项目(2018B0303110006)
广东省国际合作项目(2018A050506048)
广东省教育厅项目(2017GKTSCX057)
关键词
乌鳢
短肽
螯合肽
结构
抗氧化
Channa argus
short peptide
chelated peptide
structure
antioxidant activity
