摘要
为了探索甘油激酶(glycerol kinase,GK)基因对三角褐指藻(Phaeodactylum tricornutum)生长的影响,以GK基因过表达藻株、干扰藻株和野生型藻株为研究对象,测定了其生长情况、GK基因转录水平、GK活性、脂质含量和金藻昆布多糖等参数.结果显示:转基因藻株的比生长速率与野生型藻株无显著差异;过表达藻株GKOE1-8、GKOE7-1的转录水平显著上调,GK活性增强了3.55倍和2.58倍,中性油脂含量增加64.95%和18.49%,金藻昆布多糖含量增加50.89%和42.11%;而干扰藻株GKRNAi3-1、GKRNAi4-1的转录水平则显著下调,伴随着GK活性降低52.34%和6.64%,中性油脂含量减少26.88%和16.98%,金藻昆布多糖含量减少16.99%和10.62%.研究结果表明:在三角褐指藻中,过表达甘油激酶基因可以促进中性油脂和金藻昆布多糖等生物活性物质的积累,这为通过基因工程的手段获得产油率高的转基因藻株提供了参考.
In order to know the mechanism of glycerol kinase in Phaeodactylum tricornutum,we studied its growth,transcriptional level,enzyme activity,lipid content and chrysolaminarin content.Results showed that the growth rate of transgenic types was not significantly different from that of the control,but the transcriptional level of GK during GKOE1-8 and GKOE7-1 was significantly up-regulated.The activity of GK increased 3.55 times and 2.58 times compared with that of the control,respectively.Neutral lipid content increased by 64.95%and 18.49%.Chrysolaminarin content increased by 50.89%and 42.11%.While the transcriptional level of GKRNAi3-1 and GKRNAi4-1 were significantly down-regulated,the decrease of GK activity was 52.34%and 6.64%,respectively.The content of neutral lipid was reduced by 26.88%and 16.98%,and chrysolaminarin content decreased by 16.99%and 10.62%.The results revealed that overexpression of GK could promote the accumulation of bioactive compounds,such as neutral lipid and chrysolaminarin,and thus provide some reference for obtaining transgenic algae with high lipid through genetic engineering.
作者
高莉
张志霞
卿人韦
马力
GAO Li;ZHANG Zhixia;QING Renwei;MA Li(College of Life Science,Sichuan University,Chengdu 610064,China)
出处
《天津科技大学学报》
CAS
2019年第5期29-34,共6页
Journal of Tianjin University of Science & Technology
基金
国家自然科学基金资助项目(40976092)
四川省科技厅应用技术项目(2014JY0171)
关键词
三角褐指藻
甘油激酶
转录水平
中性油脂
金藻昆布多糖
Phaeodactylum tricornutum
glycerol kinase
transcriptional level
neutral lipid
chrysolaminarin
