摘要
[目的]建立超高压微波消解-电感耦合等离子体质谱联用测定油脂含量高的坚果食品中硒含量的方法。[方法]0.5g样品中加入4mL浓硝酸和2mL30%双氧水后,经超高压微波消解处理,采用甲烷作为反应气,用电感耦合等离子体质谱测定其中硒含量。[结果]超高压微波消解-电感耦合等离子体质谱联用法在0.1~10.0μg/L线性关系良好(R^2=0.999),方法检出限为0.4μg/kg,在5、10、50μg/kg添标水平下,回收率为93.2%~108.1%,相对标准偏差(RSD)均小于5%(n=6)。[结论]该方法准确灵敏,可以加大油脂含量高的坚果样品的称样量,提高检出限,适用于油脂含量高的坚果食品中硒含量的测定。
[Objective]The research aimed to establish a method for determination of selenium in high oil nuts with ultra-high pressure microwave-assisted and ICP-MS.[Method]Weight 0.5 g sample,added 4 mL nitric acid and 2 mL 30%hydrogen peroxide,treated by ultra-high pressure microwave(Easyprep TM),with ICP-MS to measure the contents of selenium.[Result]The proposed method had good linear relationships in the range of 0.1-10.0μg/L(R^2=0.999).The LODs was 0.4μg/kg,the average recoveries of added standards at 5,10,50μg/kg were range from 93.2%-108.1%,with the RSD less than 5%(n=6).[Conclusion]The proposed method is accurate and sensitive,it can increase the sampling amount,improve LODs of sample.It can be used for determination of selenium in high oil nuts.
作者
袁新跃
沈莉
袁燕村
周阳元
廖刘伟
季昱
陈瑜
张培洪
胡路平
刘建伟
YUAN Xin-yue;SHEN Li;YUAN Yan-cun(Food Safety Inspection Center of Fuyang District in Hangzhou,Hangzhou,Zhejiang 311400)
出处
《安徽农业科学》
CAS
2019年第17期204-206,共3页
Journal of Anhui Agricultural Sciences
基金
杭州市富阳区科技计划项目(20171226Y188)
