摘要
通过高效液相色谱法(HPLC)测定罗布麻茶黄酮中芦丁、异槲皮苷含量,并对其体外抗氧化效果进行评价。减压加热回流结合大孔吸附法提纯的罗布麻茶黄酮中芦丁和异槲皮苷实现了良好分离,其中芦丁在0.453 1~9.062 5μg时呈良好线性关系(r=0.999 55),异槲皮苷在0.258 3~5.166 5μg时呈良好线性关系(r=0.999 65),二者加标回收率分别为99.91%,100.05%(n=9),精密度试验RSD分别为0.11%,0.10%(n=5),样品中芦丁和异槲皮苷含量分别为0.059 4,0.673 4 mg/g。抗氧化试验结果表明,罗布麻茶样品清除1,1-二苯基-2-三硝基苯肼(DPPH自由基)的能力、2'-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS自由基)以及羟基自由基的能力均明显强于V C。由此可见,芦丁、异槲皮苷为罗布麻茶黄酮中最主要成分,罗布麻茶黄酮具有良好的体外抗氧化活性,是一类具有抗氧化活性的生物活性物质。
In this study,the contents of rutin and isoquercetin in flavonoids of Apocynumvenetum tea was determined by high performance liquid chromatography(HPLC),and the antioxidant effect in vitro was evaluated.A good separation of rutin and isoquercetin from flavonoids of Apocynumvenetum tea was achieved by vacuum heating reflux combined with macroporous adsorption.Rutin and isoquercetin were separated effectively in the extract of Apocynum venetum teaflavonoids.The results showed good linear relationship,the range of rutin was 0.453 1~9.062 5μg(r=0.999 55)and that of isoquercetin ranged from 0.258 3 to 5.166 5μg(r=0.999 65).The recoveries of rutin and isoquercetin were 99.91%and 100.05%(n=9).The accuracy RSD of this method were respectively 0.11%and 0.10%(n=5).The contents of rutin and isoquercetin in Apocynum venetum tea flavonoids were 0.059 4,0.673 4 mg/g respectively.Moreover,the antioxidant experimental results showed that Apocynum venetum tea flavonoids had higher scavenging abilities of 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)and hydroxyl radicals than V C positive control.It can be found that rutin and isoquercitrinwere the main components of flavonoids in Apocynumvenetum tea.The flavonoids in Apocynumvenetum tea had good antioxidant activities in vitro.
作者
张静
任小利
李希希
易沙
李冲
赵欣
ZHANG Jing;REN Xiao-li;LI Xi-xi;YI Sha;LI Chong;ZHAO Xin(Environment and Quality Inspection College,Chongqing Chemical Industry Vocational College,Chongqing 401228,China;Chongqing Collaborative Innovation Center for Functional Food,Chongqing University of Education,Chongqing 400067,China)
出处
《食品与机械》
北大核心
2019年第9期89-93,共5页
Food and Machinery
基金
重庆市教育委员会科学技术研究项目（编号：KJQN201804504）
关键词
罗布麻茶
高效液相色谱法
芦丁
异槲皮苷
抗氧化
Apocynum venetum tea
high performance liquid chromatography
rutin
isoquercetin
antioxidant
