摘要
采用超声波辅助法提取金丝皇菊总黄酮。在单因素试验的基础上,采用响应面法优化金丝皇菊总黄酮的提取工艺条件,并通过体外抗氧化试验对其抗氧化能力进行探究。结果表明:金丝皇菊总黄酮最优提取工艺参数为超声功率240 W、乙醇体积分数81%、提取时间42 min,在此条件下总黄酮得率为64.92 mg/g,金丝皇菊总黄酮提取物对DPPH·、·OH、·O_(2)^(-)和ABTS^(+)·均有显著的清除作用,且抗氧化活性与其质量浓度存在良好的量效关系。当金丝皇菊总黄酮质量浓度为0.10 mg/mL时,对DPPH·、·OH、·O_(2)^(-)和ABTS^(+)·的清除率分别为64.36%、89.42%、78.99%和63.90%,IC_(50)分别为0.058、0.036、0.043和0.043 mg/mL。
The total flavonoids from Dendranthema morifolium were extracted by ultrasonic-assisted method.On the basis of single factor experiment,the extraction conditions of total flavonoids from Dendranthema morifolium were optimized by response surface method,and its antioxidant capacity was studied by antioxidant test in vitro.The results showed that the optimum extraction parameters of total flavonoids from Dendranthema morifolium were as follows:ultrasonic power 240 W,volume fraction of ethanol 81 %,extraction time 42 min.Under these conditions,the yield of total flavonoids was 64.92 mg/g.The total flavonoids extract from Dendranthema morifolium had significant scavenging effects on DPPH;·O_(2)^(-)H,·O_(2)^(-)and ABTS^(+)·,and there was a good dose-effect relationship between antioxidant activity and its mass concentration.When the total concentration of total flavonoids in Dendranthema morifolium was 0.10 mg/mL,the scavenging rates of DPPH·,·O_(2)^(-)H,·O_(2)^(-)and ABTS^(+)· were 64.36 %,89.42 %,78.99 % and 63.90 %,respectively,and the IC_(50)was 0.058,0.036,0.043 and 0.043 mg/mL,respectively.
作者
王君
高文彬
陈新
张兆英
WANG Jun;GAO Wen-bin;CHEN Xin;ZHAGN Zhao-ying(College of Life Science,Cangzhou Normal University,Cangzhou 061001,Hebei,China)
出处
《粮食与油脂》
北大核心
2022年第9期112-117,共6页
Cereals & Oils
基金
河北省科技支撑计划项目(18226516)
河北省高等学校科学技术研究项目(Z2020248)
沧州市科学技术研究与发展重点研发计划资助项目(204203002)
沧州师范学院滨海环境生态创新团队资助项目(cxtdl1904)。
关键词
金丝皇菊
总黄酮
响应面优化
超声波辅助提取
抗氧化性
Dendranthema morifolium
total flavonoid
response surface optimization
ultrasonic-assisted extraction
oxidation resistance
