摘要
确定甘草制雷公藤的“量-毒-效”关系,探索甘草制雷公藤的安全有效的“治疗窗”,为甘草制雷公藤临床合理用药提供可靠依据。建立变应性接触性皮炎(allergic contact dermatitis,ACD)小鼠模型,进行雷公藤及甘草制雷公藤的多种剂量灌胃给药,比较肝脏生化指标(ALT,AST)、相关炎症因子(TNF-α,IL-2)的差异,以及肝脏、肾脏、脾脏、胸腺指数和耳肿胀度的差异,关联毒性指标和药效指标,综合评价在疾病模型上甘草制雷公藤的“量-毒-效”关系。结果显示,与空白对照组相比,模型组血清TNF-α,IL-2含量升高,有显著性差异(P<0.01),证明疾病模型成功,根据肝生化指标、血清炎症因子及胸腺、脾脏、肝脏、肾脏指数以及耳肿胀度综合判断该实验甘草制雷公藤剂量在3.12~12.48 g?kg^-1效果较好。研究结果显示,甘草炮制后雷公藤“治疗窗”增宽,达到了“减毒存效”效果,为临床合理安全用药提供依据。
The present study was aimed to explore the dose-toxicity-effect relationship of Tripterygium wilfordii Hook f(TW)pro-cessed by liquorice,to establish the safe and effective therapeutic window,and further to provide scientific reference for the clinical use of TW.The toxicity and anti-inflammatory effect of six doses of raw TW and TW processed by liquorice(0.78,1.56,3.12,6.24,12.48,15.60 g?kg^-1)in 1-fluoro-2,4-dinitrobenzene(DNFB)-induced allergic contact dermatitis(ACD)model were mainly exam-ined by histopathology and serum biochemistry.The liver biochemical parameters including ALT and AST,related inflammatory factors including TNF-αand IL-2,together with liver index,kidney index and the other pharmacodynamic indicators,were examined and com-pared.The results showed that compared with the control group,the serum levels of TNF-αand IL-2 of the model group were signifi-cantly increased(P<0.01),which proved that the ACD model was successful.The comprehensive analysis of liver biochemical inde-xes,serum inflammatory factors and the other indexes showed that the safe and effective therapeutic window of TW processed by liquo-rice was 3.12-12.48 g?kg^-1.The results showed the therapeutic window of TW processed by liquorice was much broader than that of raw TW.And it could provide scientific reference for the clinical rational use of TW.
作者
于小红
董捷鸣
吴昊
王伽伯
马致洁
章从恩
YU Xiao-hong;DONG Jie-ming;WU Hao;WANG Jia-bo;MA Zhi-jie;ZHANG Cong-en(Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;the Fifth Medical Center of PLA General Hospital,China Military Institure of Chinese Medicine,Beijing 100039,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第16期3454-3459,共6页
China Journal of Chinese Materia Medica
基金
首都卫生发展科研专项(首发2016-4-2024)
北京市中医药科技项目(QN2014-18)
关键词
甘草制雷公藤
变应性接触性皮炎模型
“量-毒-效”关系
治疗窗
Tripterygium wilfordii processed by liquorice
allergic contact dermatitis model
relationship of dose-toxicity-effect
therapeutic window
