摘要
目的比较低聚木糖与蔗糖对变异链球菌生长和产酸的影响,为低聚木糖在牙齿保健中的应用提供理论依据。方法分别采用质量浓度为0、1%、2%、8%、16%和20%的低聚木糖和蔗糖培养基培养变异链球菌24 h,通过Bioscreen C全自动微生物生长曲线分析仪每间隔30 min测定各组培养基的吸光度值(A值);采用pH计分别于0、5.5、10、24 h测定各组培养基的pH值,运用Origin 2017描绘其变化曲线图。比较变异链球菌培养和发酵前后各组A值及pH值的变化量(ΔA和ΔpH)。结果(1)低聚木糖组和蔗糖组细菌培养基的ΔA均随着培养时间的延长而增大(F值分别为467.165、3207.610,P<0.05);低聚木糖组和蔗糖组不同质量浓度培养基的ΔA比较,差异均有统计学意义(F值分别为11682.528、5483.421,P<0.05);且这种差异随着培养时间的延长而增大(F值分别为88.140、41.171,P<0.05)。(2)变异链球菌培养和发酵24 h后,低质量浓度(1%、2%)低聚木糖在略微或不提高变异链球菌生长水平的同时(P>0.05),明显抑制了变异链球菌的产酸能力(P<0.05);高质量浓度(16%、20%)低聚木糖在明显抑制变异链球菌生长的同时,变异链球菌的产酸水平略有提升(均P<0.05)。(3)变异链球菌培养和发酵24 h后,与同质量浓度的蔗糖相比,16%、20%的低聚木糖可明显降低变异链球菌的生长水平(P<0.05);1%、2%的低聚木糖细菌培养基的ΔA略高,但差异无统计学意义(P>0.05);8%的低聚木糖可提高变异链球菌的生长水平(P<0.05);各质量浓度低聚木糖细菌培养基的ΔpH均高于相同质量浓度的蔗糖(均P<0.05)。(4)与同甜度的蔗糖(质量浓度为8%)比较,低聚木糖(质量浓度为20%)抑制变异链球菌的生长及产酸作用更显著。结论低质量浓度低聚木糖可抑制变异链球菌产酸,高质量浓度低聚木糖可抑制变异链球菌生长;相较于蔗糖,高质量浓度低聚木糖可降低变异链球菌的生长及产酸水平,提示低聚木�
Objective To compare the effects of xylooligosaccharides(XOS)with that of sucrose on growth and acid production of cariogenic bacteria Streptococcus mutans(S.mutans),in order to provide theoretical basis and methods for further application of XOS in teeth health care.Methods S.mutans was cultured in medium containing 0,1%,2%,8%,16%and 20%XOS or sucrose,and the absorbance of the medium was determined by Bioscreen C every 30 minutes.The pH of the medium was measured at 0,5.5,10,and 24 hours using a pH meter.All the curves were plotted using Origin 2017.The changes of absorbance and pH(ΔA andΔpH)affected by the XOS or sucrose were compared before and after culture and fermentation.Results(1)ΔA of both XOS and sucrose groups increased along with the time and the difference between the two groups had statistical significance(F=467.165,3207.610,respectively,P<0.05).The difference inΔA among the different concentration groups of XOS or sucrose had statistical significance(F=11682.528,5483.421,respectively,P<0.05);the difference become bigger with time(F=88.140,41.171,respectively,P<0.05).(2)At 24 hours after culture and fermentation,the growth of S.mutans in low concentration(1%,2%)of XOS had no significant increase(P>0.05)while the acid production of it was inhibited significantly(P<0.05).The growth of S.mutans in high concentration(16%,20%)of XOS was significantly inhibited while the acid production of it was slightly increased(P<0.05)(.3)Compared to the same concentration of sucrose group at 24 hours after culture and fermentation,the growth of S.mutans in high concentration(16%,20%)of XOS was decreased(P<0.05)and that in the low concentration(1%,2%)of XOS was increased but not significantly(P>0.05).The 8%XOS could improve the growth of S.mutans(P<0.05).TheΔpH of all XOS groups were higher than that of the sucrose groups(P<0.05).(4)When the sweetness was the same,the inhibition of the growth and acid production of S.mutans XOS group(20%)was more siginificant than that of the sucrose group(8%).Conclusion High con
作者
林丹乐
谢永婷
王雪梅
LIN Dan-le;XIE Yong-ting;WANG Xue-mei(School of Stomatology,Lanzhou University,Lanzhou 730000,China)
出处
《中国实用口腔科杂志》
CAS
2019年第8期480-485,共6页
Chinese Journal of Practical Stomatology
基金
2017年兰州大学创新创业行动计划项目（20171073001565）
关键词
低聚木糖
龋病
变异链球菌
生长曲线
xylooligosaccharides
dental caries
Streptococcus mutans,S.mutans
growth curve
