摘要
[Objectives]This study was conducted to improve the efficiency of callus induction and redifferentiation,and construct high-frequency plant regeneration techniques of tissue culture in Anthuium andraeanum.[Methods]The effects of different genotypes,explant types and hormonal conditions on callus induction and re-differentiation of A.andraeanum were studied by using the aseptic A.andraeanum test-tube plantlets as test materials.[Results]Among the four kinds of aseptic A.andraeanum plantlets,the callus induction using stem segments with leaves was the best,followed by stem segments and leaves,and the petioles were the worst;among the six A.andraeanum varieties tested,the callus production rates of four varieties reached 100%;and the callus differentiation rate reached 93.3%-100%through the organogenesis pathway,and the suitable differentiation medium was 1/2MS+ZT 0.5 mg/L+2,4-D 0.1 mg/L.[Conclusions]The research results provide a new experimental basis for optimizing the technical system of A.andraeanum rapid propagation.
[Objectives] This study was conducted to improve the efficiency of callus induction and redifferentiation, and construct high-frequency plant regeneration techniques of tissue culture in Anthuium andraeanum. [Methods] The effects of different genotypes, explant types and hormonal conditions on callus induction and re-differentiation of A. andraeanum were studied by using the aseptic A. andraeanum test-tube plantlets as test materials. [Results] Among the four kinds of aseptic A. andraeanum plantlets, the callus induction using stem segments with leaves was the best, followed by stem segments and leaves, and the petioles were the worst; among the six A. andraeanum varieties tested, the callus production rates of four varieties reached 100%; and the callus differentiation rate reached 93.3%-100% through the organogenesis pathway, and the suitable differentiation medium was 1/2 MS +ZT 0.5 mg/L + 2,4-D 0.1 mg/L. [Conclusions] The research results provide a new experimental basis for optimizing the technical system of A. andraeanum rapid propagation.
基金
Supported by the Applied Basic Research Project of Suzhou City(SNG201605)
