摘要
选生长健壮的红掌盆苗,研究其组培快繁技术,结果表明：MS＋BA 1 mg/L＋NAA 0.2 mg/L＋葡萄糖30 g/L诱导率最高;1/2MS＋BA 2 mg/L＋2,4-D 0.5 mg/L＋椰汁100 mL/L＋蔗糖30 g/L培养基愈伤组织及芽苗增殖率最高;壮苗最佳培养基MS＋BA 0.5 mg/L＋IBA 0.05 mg/L＋椰汁100 mL/L＋蔗糖30 g/L;最佳生根培养基1/2MS＋NAA 0.3 mg/L＋蔗糖20 g/L,生根率达95%以上,当生根苗有3条-4条时即可移栽。
Abstract： Healthy Anthurium andraeanum seedlings planted in pot were used as materials of tissue culture. The results showed that：High induction rate was on the MS with BA 1 mg/L and NAA 0.2 mg/L and sugar 30 g/L. Callus tissue and bud-seedling multiplication rate were the highest on the 1/2MS with BA 2 rag/L＋2.4-D 0.5 mg/L ＋coconut liquid 100 mL/L＋sucrose 30 g/L;The optimum medium for strong seedling was MS＋BA 0. 5 mg/L＋ IBA 0.05 mg/L＋coconut liquid 100 mL/L＋sucrose 30 g/L. The optimum medium for rooting was 1/2MS＋NAA 0.3 mg/L＋sucrose 20 g/L. The test tube shoots rooting rate reached above 95%. The shoot with 3-4 roots will be transplanted.
出处
《山西林业科技》
2007年第3期18-19,共2页
Shanxi Forestry Science and Technology
关键词
红掌
继代培养
生根培养
移栽基质
Anthurium andraeanum
secondary culture
rooting culture
transplanting medium
