摘要
以提取胆红素后的猪胆膏余料中分离提纯的鹅去氧胆酸为原料,经酯化反应合成了6种新型的鹅去氧胆酸衍生物(3a^3f),其结构经1 H NMR,13 C NMR,IR,MS(ESI)和元素分析表征。采用滤纸片法测试了3a^3f对铜绿假单胞杆菌、大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌的抑菌活性。结果表明:用药量为2 mg·mL^-1时,3a对铜绿假单胞杆菌(d=15.3 mm),大肠杆菌(d=18.6 mm),枯草芽孢杆菌(d=11.2 mm)和金黄色葡萄球菌(d=12.2 mm)有抑制作用,3b对铜绿假单胞杆菌(d=8.0 mm)和金黄色葡萄球菌(d=10.6 mm)有抑制作用,3c对铜绿假单胞杆菌(d=10.4 mm),大肠杆菌(d=12.6 mm),枯草芽孢杆菌(d=9.7 mm)和金黄色葡萄球菌(d=15.3 mm)有抑制作用,3d对大肠杆菌(d=9.5 mm)和枯草芽孢杆菌(d=8.5 mm)有抑制作用,3e对枯草芽孢杆菌(d=9.3 mm)有抑制作用。
Six new derivatives of chenodeoxycholic acid(3a^3f)were synthesized by the esterification reaction of chenodeoxycholic acid which was purified from pig bile cream.The structures were characterized by 1H NMR,13 C NMR,IR,MS(ESI)and elemental analysis.The antibacterial activities of 3a^3f against pseudomonas aeruginosa,bacillus subtilis,escherichia coli and staphylococcus aureus were tested by filtering paper method.The results showed that 3a inhibitoried staphylococcus aureus(d=12.2 mm),pseudomonas aeruginosa(d=15.3 mm),bacillus subtilis(d=11.2 mm),escherichia coli(d=18.6 mm),3b inhibitoried pseudomonas aeruginosa(d=8.0 mm),staphylococcus aureus(d=10.6 mm),3c inhibitoried pseudomonas aeruginosa(d=10.4 mm),escherichia coli(d=12.6 mm),bacillus subtilis(d=9.7 mm),staphylococcus aureus(d=15.3 mm),3d inhibitoried escherichia coli(d=9.5 mm),bacillus subtilis(d=8.5 mm),3e inhibitoried bacillus subtilis(d=9.3 mm).
作者
王彩虹
单志炜
邓国为
张耀谋
WANG Cai-hong;SHAN Zhi-wei;DENG Guo-wei;ZHANG Yao-mou(College of Materials andEnergy,South China Agricultural University,Guangzhou 510642,China)
出处
《合成化学》
CAS
北大核心
2019年第8期638-642,共5页
Chinese Journal of Synthetic Chemistry
基金
国家级大学生创新创业训练计划项目(201810564058)
关键词
鹅去氧胆酸
酯化
合成
滤纸片法
抗菌活性
chenodeoxycholic acid
esterification
synthesis
filtering paper method
antibacterial activity
