摘要
作为遗传物质载体的染色体在细胞中并非随机分布,而是占据一定的核空间,即染色体领域(chromosome territory,CT)。染色体领域所表现出的拓扑结构及动力学特征与基因的表达,DNA的复制,DNA的损伤修复以及基因组的稳定性密切相关。传统的细胞学方法,例如荧光原位杂交(fluorescence in situ hybridization,FISH)技术,以及近些年发展起来的染色体构象捕获(chromosome conformation capture,3C)技术及其衍生技术是开展基因组构象研究的有效手段。本综述介绍了染色体空间结构的特点、不同分布模式以及3C技术的原理及其应用,希望藉此加深人们对这一领域的理解,并为植物功能基因组的研究提供借鉴。
As the genetic material carrier of chromosomes are not scattered in cells, but occupy certain nucleus space, namely, chromosome territory(CT). CT performs special topological structure and dynamic characteristics,which are closely related to the gene expression, DNA replication, DNA damage repair and genome stability.Conventional cytological methods, such as fluorescence in situ hybridization(FISH), and chromosome conformation capture( 3C) and 3C-derived technologies developed in recent years are effective means to explore genome conformation. In this review, we introduced chromosomes spatial organization characteristic, different distribution patterns, the principles and applications of 3C technologies, hoping those deepen people's understanding to this field and provide reference for the research of plant functional genomics.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第12期3501-3510,共10页
Molecular Plant Breeding
基金
国家自然科学基金(31471170
31628013)
福建农林大学人才引进启动项目(11899006004)
棉花生物学国家重点实验室开放课题(CB2015A05)共同资助
关键词
染色体领域
构象
高通量测序
荧光原位杂交
3C技术及其衍生技术
Chromosome territory
Conformation
High-throughput sequencing
Fluorescence in situ hybridization
3C and 3C-derived technologies
