摘要
本研究以红茄的子叶和下胚轴为外植体,研究其子叶和下胚轴在不同激素的浓度和组合下分化率及再生频率的变化。在诱导愈伤组织时,低浓度的NAA和高浓度的6-BA会导致下胚轴的诱导能力略低于子叶;IAA和ZT的浓度升高时,红茄下胚轴的分化率随之增加,红茄子叶的分化率随之降低。在IAA浓度为0.3 mg/L时,不定芽分化率达到最高;诱导愈伤组织的最佳培养基为MS+0.2 mg/L NAA+1.0 mg/L 6-BA;不定芽诱导最佳培养基为MS+0.3 mg/L IAA+2.0 mg/L 6-BA;不定芽生根最佳培养基为1/2MS。本研究建立的红茄再生体系,为红茄遗传转化研究奠定了基础。
In this paper, we used the cotyledon and hypocotyls of Solanum integrifolium Poir. as explants to study the changes of plant differentiation and regeneration frequency under different hormone concentrations and combinations. During callus induction, both low concentration of NAA and high concentration of 6-BA could lead to the induction of hypocotyls slightly lower than cotyledons. The hypocotyl differentiation rate increased and cotyledon differentiation rate decreased which consisted with the concentration of IAA and ZT increased. When the concentration of IAA was 0.3 mg/L, the differentiation rate of adventitious buds was the highest; The best medium for callus induction was MS+0.2 mg/L NAA+1.0 mg/L 6-BA, and for adventitious buds, the best medium was MS + 0.3 mg/L IAA + 2.0 mg/L 6-BA and the optimal rooting medium was 1/2MS. This study established a stable regeneration system of Solanum integrifolium, providing a theoretical reference for correlation study.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第10期2756-2761,共6页
Molecular Plant Breeding
基金
江苏省"六大人才高峰"高层次人才选拔培养资助项目(2015-NY-020)
江苏省苏北科技专项(BN2015099)共同资助
关键词
红茄
子叶
下胚轴
分化频率
再生频率
Solanum integrifolium Poir.
Cotyledon
Hypocotyl
Differentiation rate
Regeneration rate
