摘要
根腐病是菠萝蜜生产中的重要病害之一,但其病原未见相关报道。为确定菠萝蜜根腐病病原,2013-2014年从海南省11个市县采集菠萝蜜病根样品15份,按照常规分离方法,获得62个分离物,对出现频率最高的优势菌采用柯赫氏法鉴定其致病性,应用形态学和PCR方法鉴定分离物种类,采用菌落生长法和玻片法研究其部分生物学特性。结果表明,62个分离物,经形态学初步鉴定归属于11个属(种),致病性测定结果显示引起菠萝蜜根部腐烂病害的优势致病菌为腐皮镰刀菌(Fusarium solani),不同培养基对病原菌菌丝生长的影响差异明显,其最佳培养基为菠萝蜜幼果煎汁培养基;温度、p H值、碳氮源对菌丝生长差异显著,菌丝生长最佳温度为28℃,菌丝的致死温度为63℃10 min,孢子最佳萌发温度为25℃,菌丝生长最佳p H值为7,最佳碳源为葡萄糖,最佳氮源为硝酸铵。根腐病是菠萝蜜生产中的重要病害之一,但其病原未见相关报道,这是首次关于腐皮镰刀菌(Fusarium solani)导致菠萝蜜根腐病在我国发生和研究的报道,通过此研究以期为该病害的有效防控提供理论依据。
The disease roots o f Artocarpus heteophyllus Lam with root rot symptom were sampled in Hainan,China. Sixty-two fungal isolates were finally from 15 samples of jackfruit root rot collected from 11 cities. The pathogen of the predominant fungus from the samples of diseased tissues was tested according to Koch's law, the Pathogen was identified according to morphological Characteristics and ribosomal DNA internal transcribed spacer(r DNA-ITS) sequence analysis, biological characteristics were using colony growth and slide methods. Among them, the results showed that the representative isolate was identified Fusarium solani. Effects of different cultures on mycelia growth were significant, optimal culture for mycelia growth was young-fruit decocted with liquid of jackfruit; effects of temperature, p H, carbon and nitrogen sources on mycelia growth and spore germination had significant differences, optimal temperature for mycelia growth was 28℃, fatal temperature for mycelia growth was 63℃, 10 minutes; optimal temperature for spore germination was 25℃; p H7 was the optimal PH for mycelia growth; the optimal carbon source for mycelia growth was dextrose, ammonium nitrate was the optimal nitrogen sources for mycelia growth. This is the first report of root rot caused by F. solani on Artocarpus heteophyllus Lam.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第8期2205-2212,共8页
Molecular Plant Breeding
基金
海南省省属科研院所技术开发专项项目(KYYS-2016-09)
海南省农业科技110建设项目(HNNYKJSJ201546)
农业部热带作物种质资源保护项目(16RZZY-10)
国家热带作物种质资源平台(菠萝蜜种质资源整理,整合及共享运行服务)项目共同资助
关键词
菠萝蜜
根腐
病原菌鉴定
生物学特性
Artocarpus heteophyllus Lam
Root rot
Pathogen identification
Fusarium solani
Biological characteristic
