摘要
为掌握禾谷镰孢在甘肃中部地区的分布及变异情况,从根部表现有坏死和叶鞘发褐的小麦幼苗的不同部位.小麦地土壤及玉米籽粒.玉米茎秆上分离禾谷镰孢,并以形态学为基础,参照Nelson分类系统进行鉴定.结果表明,在分离到的43个镰刀菌菌株中,有14 个菌株经鉴定为禾谷镰孢,均从玉米茎秆上分离到,小麦根部.小麦叶鞘.小麦地土壤.玉米籽粒中分离到的镰刀菌中未见禾谷镰孢.将禾谷镰孢在特定条件下培养后,发现14 个禾谷镰孢菌株产生子囊壳的数量不同,为2-90个.在以Fg16为引物的PCR 反应中,随机选取的11个禾谷镰孢菌株都产生0.41kb的PCR 产物,而6个对照菌株都产生0.50kb的片段,证明引物Fg16可以区分禾谷镰孢菌株群体的遗传多态性.以Tri13为引物的PCR 反应显示,11个禾谷镰孢菌株以及3 个中国对照菌株都产生脱氧雪腐镰刀菌烯醇（DON）毒素,而3个澳大利亚对照菌株产生雪腐镰刀菌烯醇（NIV）毒素.
The occurrence of Fusarium graminearum on Triticum aestivurn and Zea mays, in the middle areas of Gansu Province, China was assessed by isolation of Fusariurn species from T. aestivum roots with brown rot symptoms, from T. aestivum sheaths, T. aestivurn soil, Z. rnays kernels, and Z. mays stalks. Fusarium isolates were identified by morphology according to Nelson＇s system. Fourteen of 43 Fusarium isolates were F. graminearurn. It was isolated only from corn stalks. These 14 isolates produced between 2 and 90 peritheeia each. PCR using the Fgl6 primer set resulted in a product of 0.41 kb from all 14 isolates and of 0.50 kb from the 6 reference isolates. It successfully differentiated between the F. graminearurn isolates. PCR reactions using the Tri13 primer showed that all 14 isolates and the three Chinese reference cultures produced DON while the three Australian reference isolates produced fragments of 415 bp showing that they produced NIV.
出处
《草业学报》
CSCD
北大核心
2009年第1期118-124,共7页
Acta Prataculturae Sinica
基金
澳大利亚ATSE/Crawford基金
甘肃省科技厅(甘科计[2001]21号)
甘肃省教育厅项目(032208)资助
