摘要
目的 :建立高效液相色谱法 (HPLC)测定宫炎康颗粒中芍药苷的含量。方法 :采用HPLC法 ,VYDAC2 18TP5 4柱 (4 .6mm× 15 0mm ,5 μm) ;乙腈 0 .1%磷酸溶液 (15∶85 )为流动相 ;检测波长 2 43nm。 结果 :芍药苷在0 .0 982～ 0 .785 6μg范围内线性关系良好 ,r =0 .9997,回收率 10 3 .7% ,RSD =2 .6%。 结论 :该方法方便、准确、可控 ,可作为该产品的定量控制方法。
Objective:To establish a HPLC method for determination of paeoniflorin in gongyankang granule. Method:The content of paeoniflorin was determined by HPLC, using VYDAC218TP54 column; acetonitrile-0.1% phosphoric acid(15∶85) as mobile phase; The detective wavelength was 243 nm. Results:Paeoniflorin at the concentrations of 0.098 2-0.785 6 μg showed good linearity ( r =0.999 7). The average recovery was 103.7% with RSD 2.6%. Conclusion:The results show that the method is simple and accurate. It can be useful in quality control of the products.
出处
《医药导报》
CAS
2003年第1期55-56,共2页
Herald of Medicine
关键词
中药
高效液相色谱法
宫炎康颗粒
芍药苷
含量
High perfarmance liguid chromatography
Gongyankang granule
Paeoniflorin
Determination
