摘要
海洋红酵母是一种优质的天然海参饵料。研究旨在提高海洋红酵母(oceanic red yeast,ORY)菌浓度和虾青素的含量水平,以降低ORY培养成本。从渤海海域筛选出一株ORY,用麦汁培养基对ORY富集培养,并进行菌种鉴定。通过对温度、不同转速、接种量、p H值、番茄红素的添加量及添加时间的单因素试验,以及p H值、番茄红素的添加量及添加时间三因素三水平的响应曲面试验,以优化ORY的菌体生长以及虾青素积累的培养条件。经过优化后的培养条件为:温度30℃、初始p H值4.99、转速180 r/min、接种量10%、在菌种发酵27.25 h投放番茄红素62μg。在此条件下ORY的细胞数达到14.82×108CFU/ml,虾青素含量达到1 571.00μg/g,与传统培养方式相比分别提高了64.12%和86.14%,从而使得ORY的产量以及质量均有提高,同时降低了其生产成本。
Oceanic red yeast is a kind of high-quality natural bait for the sea cucumber.This studyaimed to improve the oceanic red yeast(oceanic red yeast, ORY) concentration and astaxanthin outputlevels and reduce the ORY training costs. ORY was obtained from the Bohai Sea and enriched for spe-cies identification with the wort medium.Through the single factor experiment of the temperature, thep H, the different speed, the inoculation quantity, lycopene addition amount and the adding time aswell as three factors three levels of response surface experiments of the p H, the content of lycopeneand the adding time, we have found the most beneficial to the ORY growth and astaxanthin productionof culture conditions. The optimized culture conditions were as follows: temperature 30 ℃, initial p H4.99, speed 180 r/min, quantity of 10%, addion lycopene 62 μg when bacteria fermentated for 27.25 h.Under this optimized condition, the number of ORY has enhanced up to 14.82×108CFU/ml with thenumber of astaxanthin up to 1 571.00 μg/g, up 64.12% and 86.14% respectively compared with thetraditional conditions. Results indicate that with the ORY optimized wort medium culture, ORY yieldand quality has improved, then the production cost is reduced.
出处
《饲料工业》
北大核心
2015年第20期51-56,共6页
Feed Industry
关键词
海洋红酵母
虾青素
发酵
条件优化
oceanic red yeast
astaxanthin
fermentation
condition optimization
