摘要
探讨高血压大鼠基底动脉结构的变化及血管紧张素转换酶抑制剂对其的影响。选用易卒中型肾血管性高血压大鼠模型 ,分为高血压组、卡托普利治疗组、假手术正常血压对照组。分别于肾动脉狭窄术后 4、8、12周处死动物 ,取基底动脉 ,制片后分别用光镜和透射电镜观察 ,并进行体视学定量分析。术后 4周时高血压组基底动脉光镜下无明显形态学变化 ,8周和 12周时中膜厚度、壁腔比值均比同时期正常血压对照组增加 ,有显著性差异 (P <0 .0 5 ) ;卡托普利治疗后中膜厚度、壁腔比值均小于高血压组 ,差异有显著性 (P <0 .0 5 )。术后 4周高血压组基底动脉平滑肌细胞间隙略增宽 ;术后 8周细胞器肿胀 ,部分溶解 ,间质轻度水肿 ;12周时平滑肌细胞肌丝变性、断裂、溶解 ,内质网扩张 ,线粒体部分空泡变性 ,细胞坏死。卡托普利组各时期超微结构改变不明显。表明高血压可致基底动脉肥厚和超微结构破坏 ,而卡托普利可以预防高血压所致的基底动脉结构破坏。
Aim To study the structural changes of basilar arteries during hypertension and the effects of captopril. Methods The animal model of stroke prone renovascular hypertensive rats might be divided into two groups, hypertension group and captopril treated group; one sham operated group was used asnormotensive control group. Each group took the observation of the structure of the basilar arteries under light microscope and transmission electrical microscope as well as analyses of morphometry at 4, 8, 12 weeks respectively. Results There were no obvious microscopic changes in basilar arteries of the hypertension group postoperation 4 weeks. The media thickness and wall to lumen ratio of the hypertension group were higher than those of captopril and sham operated groups (P<0.05). The intercellular spaces are broadened slightly at 4 weeks after operation; there were moderate ultrastructural damages at the end of 8 weeks, with the organelle oedema and partial lysis, interstitial dropsy; there were obvious ultrastructural damages at the end of 12 weeks, such as smooth muscle cell myofilament degeneration, fragmentation, lysis, and endoplasmic reticulumnecrosis dilataltion, mitochondria vacuolation, smooth muscle cell necrosis. The ultrastructure appearances of the captopril treated group were nearly normal. Conclusions Hypertension can cause hypertrophy and ultrastructural damage of basilar arteries. Captopril can prevent media hypertrophy of basilar arteries in experimental hypertensive rats and protect the ultrastructure against injury factors.
出处
《中国动脉硬化杂志》
CAS
CSCD
2002年第5期392-395,共4页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金 (3 9970 849)
广东省自然科学基金团队项目 (2 0 0 0年 )
广东省自然科学基金 (990 0 65 )
美国CMB基金(0 0 -73 0 )资助
