摘要
采用玫瑰花结抑制试验方法观察了BALB/c小鼠受精卵培养液经氯仿提取前后早孕因子(EPF)样活性的变化。结果显示,未经氯仿提取的受精卵培养液表现有与早孕血清相似的EPF样活性,但经氯仿提取后EPF样活性消失,而早孕妇女血清中的EPF活性不受氯仿提取的影响。提示,导致小鼠受精卵培养液中EPF样活性表达的物质是高脂溶性的,在化学本质上不同于人早孕血清中的EPF,由受精卵释放的某种高脂溶性物质(可能是胚源性血小板活化因子EDPAF)作用于血小板(或其它成分),使之释放出EPF。
The change of early pregnancy factor(EPF)—like activities in the BALB/c mouse fertilized ovum culture fluid before and after chloroform extraction was observed by means of the rosette inhibition test in vitro. The results revealed that the culture fluid beforeextraction displayed the activity of EPF—like similar to that of early pregnancy sera, and that culture fluid after extraction by chloroform did not display this activity but the EPF—activity of the pooled early pregnancy sera after extraction still existed. It suggests that the substances of expressing EPF—like activity in the fertilized ovum culture fluid are highly lipophilic and differ chemically from the EPF in human early pregnancy sera. The expression of EPF—like activity in the mouse fertilized ovum culture fluid is quite possibly due to the high lipophilic substance(like EDPAF) released by mouse fertilized ovum acting on the platelets(or other compositions) and the lalter release EPF.
出处
《安徽医科大学学报》
CAS
1992年第2期102-104,共3页
Acta Universitatis Medicinalis Anhui
基金
国家"七五"攻关课题
关键词
氯仿
PAF
早孕因子
生殖免疫学
chloroform
zygote
rosette formation/methods, platelet activating factor (PAF)
mice
