摘要
目的:研究海洋贝类提取物（EMS）对动脉粥样硬化（AS）形成的抑制作用。方法:建立高脂食饵性鹌鹑AS模型,采用酶法测定主动脉和心肌中总胆固醇（TC）和甘油三酯（TG）含量,并对主动脉、冠状动脉及肝脏进行肉眼及光镜检查,对AS病变及肝脏脂肪变性程度进行分级比较。结果:EMS 20g、10g、5g·kg-1（生药）经连续灌胃给药8周和11周时,动物的主动脉、心肌组织中TC及TG含量均明显低于模型组（P<0.05,P<0.01）。病理组织学检查结果显示,与模型组比较,EMS各组动物的立动脉和冠状动脉的AS病变程度较轻,多数在I级以下,AS面积小,内膜厚度及内膜下层的泡沫细胞及平滑肌细胞数目明显减少。肝脏脂肪变性程度较模型组减轻。结论:EMS预防用药可抑制实验性AS形成。
Aim To research the inhibitory action of the extract from maritime shellfish on formation of atherosclerosis. Method The model of experimental atherosclerosis in quails was established by hyper lipoidal feed. The content of total cholersterol (TC) and triglycer-ides(TG) in aortas and cardiac muscle were detected with enzyme method. The atherosclerotic plaque lesion in aortic and conorary artery and liver fatty degeneration were observed by eye and through light microscope. Results EMS 20,10,5g · kg-1 ig significantly reduced the levels of TC and TG in aorta and cardiac muscle compared with model group at 8 and 11 wk (P<0. 05,P<0. 01). The degree of atheroscleroti lesion in aortic and coronary arterious en-dothelium in EMS group were lightened and most of them were slighter than one grade and the scope of atherosclerotic plaque was reduced (P<0. 05,P<0. 01) . Administration of EMS resuled in a marked decreased, the intimal thickness and amount of foam and smooth muscle cells in subendothelium. The degree of live fatty degeneration in EMS group was lower than model group. Conclusions The results suggested that preventive administration of EMS has an inhibitory effect on the development of experimental atherosclerosis.
出处
《中国海洋药物》
CAS
CSCD
2002年第5期18-21,共4页
Chinese Journal of Marine Drugs
基金
山东省省科委课题资助(项目编号:981164805)
国家自然科学基金资助(项目编号:30171157)
