摘要
四列藻被认为是介导广盐性轮虫有性生殖的优质饵料。然而,新鲜的四列藻培养成本高且营养单一难以维持轮虫的高密度、高质量培养。因此,寻找一种营养素含量高、经济效益好,且能提高轮虫增殖效率的商业化饵料对水产养殖业的发展有重要意义。本研究通过比较四列藻和富硒小球藻对褶皱臂尾轮虫复合品系(日本品系和澳大利亚品系)的生殖及休眠卵孵化率的影响,探究了富硒小球藻饵料在轮虫高密度培养中的可用性。研究发现,日本品系轮虫,富硒小球藻组的休眠卵孵化率(70.0±11.1)%显著高于四列藻组(24.0±10.8)%(p<0.05);而澳大利亚品系,虽然休眠卵孵化率在两种饵料间无显著性差异,但小球藻组的种群生长率(0.28±0.02)高于四列藻组(0.23±0.00)(p<0.05)。由此可见,富硒小球藻有利于褶皱臂尾轮虫复合品系的种群增长和有性繁殖。此外,富硒小球藻饵料组轮虫的体长和体宽均小于四列藻组,这一结果也证明了轮虫种群密度越大,体型越小的理论。本研究结果显示在褶皱臂尾轮虫复合品系的高密度培养中,商业化的富硒小球藻有望替代四列藻成为一种有效的提高轮虫有性生殖效率的饵料。
Tetraselmis tetrathele is an optimal diet to induce sexual reproduction of euryhaline rotifers. However, the culture cost of fresh T. tetrathele is high and the nutrition is single, it is difficult to maintain the high-density and high-quality rotifer. Hence, to find a commercial diet which has high nutrition and can increase the rotifer reproduction efficiency is important for the flourishing development of aquatic industry. This study investigated the efficiency of commercial Chlorella vulgaris in the mass culture of rotifer by comparing their reproduction and resting egg hatchability with T. tetrathele. The result shows that when the Japanese strain, Brachionus plicatilis sensu stricto fed with two different diets, the produced resting eggs showed higher hatching rate with C. vulgaris ((70.0±11.1)%, p<0.05) than T. tetrathele (24.0±10.8)%. For the Australia strain, the hatchability of resting eggs is no significant difference between the two diets, while, Brachionus manjavacas showed higher population growth rate (r) with C. vulgaris (0.28±0.02, p<0.05) than with T. tetrathele (0.23±0.00). Hence, the commercial selenium enriched chlorella is beneficial to the population growth and sexual reproduction of Brachionus plicatilis species complex. Furthermore, the body length and width of rotifers fed with C. vulgaris diet were lower than with T. tetrathele, this result confirmed that the population density of rotifer influence its body size. The results of our study indicates that the Chlorella vulgaris is considered as an efficient candidate for T. tetrathele to improve the sexual reproduction efficiency of rotifer in the mass culture of B. plicatilis species complex.
作者
韩程燕
金禧珍
萩原篤志
李名友
Han Chengyan;Kim Hee-Jin;Hagiwara Atsushi;Li Mingyou(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai,201306;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai,201306;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai,201306;Graduate School of Fisheries and Environmental Sciences,Nagasaki University,Nagasaki,852-8521)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第9期4013-4019,共7页
Genomics and Applied Biology
基金
国家自然科学基金项目(31672700)
上海市曙光计划项目(SG42)
日本学术振兴会(JSPS)科研项目(JP17H03862)共同资助
关键词
轮虫
四列藻
富硒小球藻
生殖
休眠卵孵化
Rotifera
Tetraselmis tetrathele
Chlorella vulgaris
Reproduction
Resting egg hatching
