摘要
目的研制基于上转换颗粒(up-conversion particles,UCNPs)和适配体探针的试纸条,检测一次性纸杯热溶出的双酚A(bisphenol A,BPA)。方法制备高发光效率的UCNPs,对其表面进行氨基和链霉亲和素修饰并连接适配体制备荧光探针,通过优化加样体积和硝酸纤维素膜(cellulose nitrate membrane,NC)种类成功组装试纸条。结果未修饰的UCNPs粒径约为73 nm,表面成功修饰氨基和链霉亲和素之后的粒径约为85 nm,在测试样品体积为200μL,以Sartorius CN 140作为硝酸纤维素膜的最优检测条件下,试纸检测范围为50~800 ng/mL,最低检出限达12.5 ng/mL。该方法应用于纸杯溶出的BPA检测,加标回收率在94.53%~103.78%之间,相对标准偏差小于3.75%,与国家标准高效液相色谱法的测得结果无显著性差异(P>0.05)。结论用本实验研制的试纸条检测一次性纸杯热溶出的BPA,灵敏度高、特异性好、检测时间短,适用于现场检测。
Objective To develop a test strip based on UCNPs and aptamer probes,and test the BPA dissolved from disposable cups.Methods The UCNPs with high luminescence efficiency were prepared and modified with amino-group and streptavidin on its surface.Then,the fluorescent probes were prepared by connecting the aptamer.The test strip was successfully assembled by optimizing the amount of sample added and the type of cellulose nitrate membrane.Results Diameter of bare UCNPs was about 73 nm,after surface modification with amino-group and streptavidin,the diameter increased to about 85 nm.Under the optimal detection condition of Sartorius CN 140 as the cellulose nitrate membrane,with the sample volume of 200μL,the detection range of strip for BPA was from 50 ng/mL to 800 ng/mL and the limit of detection was 12.5 ng/mL.The method was applied to detect the BPA dissolved from paper cups.The recoveries were between 94.53%and 103.78%,the RSD was below 3.75%and there was no significant difference(P>0.05)between the results obtained by the HPLC from national standard.Conclusion The test strip developed in this experiment can be used to detect the BPA dissolved from the disposable paper cup,which has high sensitivity,good specificity and short detection time,and is suitable for on-site detection.
作者
李巧凤
白家磊
彭媛
王周平
高志贤
LI Qiao-Feng;BAI Jia-Lei;PENG Yuan;WANG Zhou-Ping;GAO Zhi-Xian(Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety,Institute of Environmental and Operational Medicine,Academy of Military Medical Science,Academy of Military Science,Tianjin 300050,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
出处
《食品安全质量检测学报》
CAS
2019年第18期6223-6227,共5页
Journal of Food Safety and Quality
基金
国家重点研发计划项目(2017YFC1200903)
天津市科技支撑计划项目(18YFZCNC01260)~~
关键词
上转换颗粒
适配体
双酚A
试纸条
up-conversion particles
aptamer
bisphenol A
test strip
