摘要
为探究根系分泌物对铝胁迫下栝楼根部的影响,实验选取浦江栝楼和安国栝楼为材料,在土培条件下观察不同浓度根系分泌物对铝处理下栝楼根系生长特性和细胞壁官能团的影响.结果表明:铝胁迫对2种栝楼的生长均有一定抑制,各指标在低浓度根系分泌物施用后得到恢复,但随着浓度上升呈现先升后降的趋势;细胞壁红外光谱图显示,R-O振动吸收峰和-OH振动吸收峰在处理下升高,同时也证明-NH,C=O,C==C,-CH2官能团是中和铝毒或螯合Al3+的重要官能团;各处理组中,Y300-20的红外吸收峰最接近对照组,说明低浓度根系分泌物维持了细胞壁物质的稳定,而高浓度根系分泌物会导致自毒效应.研究结果为铝毒机理研究和铝胁迫缓解途径的探求提供了依据.
It was aimed to investigate the effects of root exudates on the roots of Trichosanthes kirilowii under aluminum stress,both Anguo T.kirilowii and Pujiang T.kirilowii were used as experimental materials.The effects of different concentrations of root exudates on the growth characteristics and cell wall functional groups of roots under aluminum stress were investigated by soil culture method.Al stress inhibited the root growth of two varieties of T.kirilowii,it could be recovered after root exudates were applied.However,each index showed a trend of rising and then decreasing with the increasing of concentration of root exudates.By FT-IR spectral study,-OH stretching and R-O stretching increased after aluminum treatment.In addition to the aluminum resistance mechanism,-NH,C=O,C==C,-CH2 functional groups played an important role in neutralizing Al toxicity or chelating Al 3+.Among all groups,Y 300-20 showed the same absorption peak to CK,indicated that the root exudates could effectively protect the cell wall components,but high concentrations of root exudates can lead to self-toxic effects.The results provide a basis for the investigation of the mechanism of aluminum toxicity and the exploration of aluminum stress alleviation pathways.
作者
马丽
沈蔡慰
陈瑞
吴玉环
崔乐怡
陶涛
张婷婷
朱叶心怡
刘鹏
MA Li;SHEN Caiwei;CHEN Rui;WU Yuhuan;CUI Leyi;TAO Tao;ZHANG Tingting;ZHU Yexinyi;LIU Peng(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China;College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310036,China)
出处
《浙江师范大学学报（自然科学版）》
CAS
2019年第4期414-420,共7页
Journal of Zhejiang Normal University:Natural Sciences
基金
国家自然科学基金资助项目(41571049
30540056)
浙江省公益技术应用资助项目(2011C22053)
关键词
栝楼
铝胁迫
根系分泌物
细胞壁
官能团
Trichosanthes kirilowii
aluminum stress
root exudates
cell wall
functional group
